This mAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.
Aufreinigung
Protein G affinity chromatography
Immunogen
A partial recombinant protein (aa 82-238) was used as the immunogen for the CELA3B antibody.
Optimal dilution of the CELA3B antibody should be determined by the researcher.\. ELISA : 2-4 μg/mL, order BSA free format for coating,Western blot: 1-2 μg/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.2 mg/mL
Buffer
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store the CELA3B antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
CBPP antikoerper, E1 antikoerper, EL-1 antikoerper, ELA3B antikoerper, 0910001F22Rik antikoerper, 2310074F01Rik antikoerper, AI504000 antikoerper, Ela3 antikoerper, Ela3b antikoerper, ela3b antikoerper, MGC82867 antikoerper, ELA1 antikoerper, Cela3b antikoerper, CELA3B antikoerper, chymotrypsin like elastase family member 3B antikoerper, chymotrypsin-like elastase family, member 3B antikoerper, elastase 3B, pancreatic antikoerper, proproteinase E antikoerper, chymotrypsin like elastase family member 3B L homeolog antikoerper, chymotrypsin-like elastase family member 3B antikoerper, CELA3B antikoerper, Cela3b antikoerper, ela3b antikoerper, LOC478196 antikoerper, cela3b.L antikoerper, LOC100717936 antikoerper, LOC100071692 antikoerper
Hintergrund
This mAb recognizes a protein of ~30 kDa, identified as CELA3B (Chymotrypsin like elastase family member 3B), or Elastase 3B. Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six genes which encode the structurally similar proteins: Elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, 3B has little elastolytic activity. Like most of the human elastases, 3B is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. It may also function in the intestinal transport and metabolism of cholesterol. Elastases 3A/B have been referred to as Protease E and as Elastase 1, and excretion of this protein in fecal material is frequently used as a measure of pancreatic function in clinical assays.