Keratin Basic Antikörper

Produktnummer ABIN3023993

Produktdetails

Target: Keratin Basic

Reaktivität: Human, Ratte, Maus, Kaninchen, Rind (Kuh), Huhn, Affe, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G purified

Immunogen: Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.

Klon: AE3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Differences in protocols and secondaries may cause the Basic Cytokeratin antibody to need titering for optimal performance.

1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Antigendetails

Target: Keratin Basic

Andere Bezeichnung: Basic Cytokeratin (Keratin Basic Produkte)

Hintergrund: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 52  kDa (CK8). Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).

Gen-ID: 3848