pan Keratin Antikörper

Produktnummer ABIN3023997

Produktdetails anti-pan Keratin Antikörper

Target: pan Keratin (panKRT)

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Affe, Hund, Kaninchen, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser pan Keratin Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: Human epidermal keratin was used as the immunogen for this pan Cytokeratin antibody.

Klon: AE1-AE3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody AE1 + AE3 to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,for 2 hours at RT,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the pan Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu pan Keratin

Target: pan Keratin (panKRT)

Andere Bezeichnung: Pan Cytokeratin (panKRT Produkte)

Synonyme: xk70a antikoerper, keratin antikoerper, keratin S homeolog antikoerper, LOC431321 antikoerper, krt.S antikoerper

Hintergrund: Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This pan keratin antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3-6, CK8, CK10, CK14-16, and CK19. Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The AE1 + AE3 antibody cocktail is a broad spectrum pan cytokeratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of keratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. It stains cytokeratin present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.