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Melanoma Antikörper

Reaktivität: Human, Hund, Maus IHC (p), IF Wirt: Maus Monoclonal PNL2 unconjugated
Produktnummer ABIN3024167
  • Target Alle Melanoma Produkte
    Melanoma
    Reaktivität
    • 4
    • 2
    • 1
    • 1
    Human, Hund, Maus
    Wirt
    • 4
    Maus
    Klonalität
    • 4
    Monoklonal
    Konjugat
    • 4
    Dieser Melanoma Antikörper ist unkonjugiert
    Applikation
    • 3
    • 3
    • 2
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Produktmerkmale
    Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87 % ), as opposed to 76 % for anti-HMB45 and 82 % for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF.
    Reinheit
    Protein G affinity chromatography
    Immunogen
    Melanocyte antigen was used as the immunogen for the Melanoma antibody PNL2.
    Klon
    PNL2
    Isotyp
    IgG1
  • Applikationshinweise
    Optimal dilution of the Melanoma antibody PNL2 should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    1 mg/mL in sterile PBS, BSA free, sodium azide free
    Konservierungsmittel
    Azide free
    Lagerung
    -20 °C,4 °C
    Informationen zur Lagerung
    Store the Melanoma antibody PNL2 at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Melanoma
    Abstract
    Melanoma Produkte
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