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Melanoma Antikörper

Reaktivität: Human, Pferd IHC (p), IF, FACS Wirt: Maus Monoclonal NKI-beteb unconjugated
Produktnummer ABIN3024241
  • Target Alle Melanoma Produkte
    Melanoma
    Reaktivität
    • 4
    • 2
    • 2
    • 1
    Human, Pferd
    Wirt
    • 4
    Maus
    Klonalität
    • 4
    Monoklonal
    Konjugat
    • 4
    Dieser Melanoma Antikörper ist unkonjugiert
    Applikation
    • 3
    • 3
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
    Keine Kreuzreaktivität
    Ratte (Rattus)
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    Membranes from a human melanoma metastasis were used as the immunogen for this Melanoma antibody (gp100).
    Klon
    NKI-beteb
    Isotyp
    IgG2b kappa
  • Applikationshinweise
    Differences in protocols and secondaries may require the gp100 antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Konservierungsmittel
    Azide free
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the Melanoma antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Melanoma
    Abstract
    Melanoma Produkte
    Hintergrund
    By immunohistochemistry, this melanoma gp100 antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. The melanoma antibody labels formalin/paraffin melanomas and other tumors showing melanocytic differentiation.
    Gen-ID
    6490
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