Glypican 3 Antikörper
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- Target Alle Glypican 3 (GPC3) Antikörper anzeigen
- Glypican 3 (GPC3)
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Reaktivität
- Human, Ratte
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Glypican 3 Antikörper ist unkonjugiert
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Applikation
- Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- Recombinant full-length human GPC3 protein was used as the immunogen for the Glypican-3 antibody.
- Klon
- GPC3-863
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product GPC3 Primärantikörper
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- Applikationshinweise
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Optimal dilution of the Glypican-3 antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2) - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store the Glypican-3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- Glypican 3 (GPC3)
- Andere Bezeichnung
- Glypican-3 (GPC3 Produkte)
- Synonyme
- GPC3 antikoerper, sgb antikoerper, dgsx antikoerper, sdys antikoerper, sgbs antikoerper, oci-5 antikoerper, sgbs1 antikoerper, DGSX antikoerper, GTR2-2 antikoerper, MXR7 antikoerper, OCI-5 antikoerper, SDYS antikoerper, SGB antikoerper, SGBS antikoerper, SGBS1 antikoerper, Glypican-3 antikoerper, glypican 3 antikoerper, gpc3 antikoerper, GPC3 antikoerper, Gpc3 antikoerper
- Hintergrund
- Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100 % in follicular carcinoma and 70 % in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
- Pathways
- Glycosaminoglycan Metabolic Process
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