TOX3 Antikörper (AA 200-400)

Produktnummer ABIN3025030

Produktdetails anti-TOX3 Antikörper

Target: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Bindungsspezifität: AA 200-400

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TOX3 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktmerkmale: It recognizes a 63 kDa protein, which is identified as TOX3. It contains a high mobility group (HMG)-box, which regulates Ca2+-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB). TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts.

Aufreinigung: Protein G affinity chromatography

Immunogen: A recombinant fragment (139 amino acid residues around aa 200-400) from the human protein was used as the immunogen for the TOX3 antibody.

Klon: TOX3-1123

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the TOX3 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0 or 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the TOX3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu TOX3

Target: TOX3 (TOX High Mobility Group Box Family Member 3 (TOX3))

Andere Bezeichnung: TOX3 (TOX3 Produkte)

Synonyme: TNRC9 antikoerper, cagf9 antikoerper, tnrc9 antikoerper, CAGF9 antikoerper, 500-9 antikoerper, BC052044 antikoerper, C230068E13 antikoerper, Tnrc9 antikoerper, TOX high mobility group box family member 3 antikoerper, TOX3 antikoerper, tox3 antikoerper, Tox3 antikoerper

Hintergrund: It recognizes a 63  kDa protein, which is identified as TOX3. It contains a high mobility group (HMG)-box, which regulates Ca2+-dependent transcription in neurons through interaction with the cAMP-response-element-binding protein (CREB). TOX3 appears to be associated with breast cancer susceptibility and is expressed downstream of a cytoprotective cascade together with CITED1, a transcriptional regulator that does not bind directly to DNA. TOX3 is predominantly expressed in the brain and forms homodimers. TOX3 overexpression protects neuronal cells from cell death caused by endoplasmic reticulum stress or BAX overexpression through the induction of anti-apoptotic transcripts and repression of pro-apoptotic transcripts.

Pathways: Chromatin Binding