MFGE8 Antikörper

Produktnummer ABIN3025866

Produktdetails anti-MFGE8 Antikörper

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MFGE8 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: Protein G affinity chromatography

Immunogen: A human milk fat globule membrane preparation was used as the immunogen for the MFGE8 antibody.

Klon: MFG-06

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the MFGE8 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 1-2 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the MFGE8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu MFGE8

Target: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Andere Bezeichnung: MFGE8 (MFGE8 Produkte)

Hintergrund: Recognizes a protein of 40-45  kDa, identified as human milk fat globule membrane protein (HMFG), Lactadherin, or MFG-E8. HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.

Pathways: SARS-CoV-2 Protein Interaktom