CD43 Antikörper
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- Target Alle CD43 (SPN) Antikörper anzeigen
- CD43 (SPN) (Sialophorin (SPN))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD43 Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Produktmerkmale
- This antibody recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. 70-90 % of T-cell lymphomas and 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with CD20 antibody, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with CD20 and CD42 antibody argues against a reactive process and favors a diagnosis of lymphoma.
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- Myeloblastic KG1 cells were used as the immunogen.
- Klon
- DF-T1
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product SPN Primärantikörper
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- Applikationshinweise
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western Blot: 0.5-1 μg/mL, FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store the CD43 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- CD43 (SPN) (Sialophorin (SPN))
- Andere Bezeichnung
- CD43 (SPN Produkte)
- Synonyme
- CD43 antikoerper, GALGP antikoerper, GPL115 antikoerper, LSN antikoerper, A630014B01Rik antikoerper, Cd43 antikoerper, Galgp antikoerper, Ly-48 antikoerper, Ly48 antikoerper, Lsn antikoerper, Lsn1 antikoerper, gpL115 antikoerper, sialophorin antikoerper, SPN antikoerper, Spn antikoerper
- Hintergrund
- This antibody recognizes a cell surface glycoprotein of 95/115/135 kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. 70-90 % of T-cell lymphomas and 22-37 % of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with CD20 antibody, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with CD20 and CD42 antibody argues against a reactive process and favors a diagnosis of lymphoma.
- Gen-ID
- 6693
- Pathways
- Regulation of Leukocyte Mediated Immunity
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