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Actin, Muscle Specific Antikörper

Reaktivität: Human, Kaninchen, Ratte FACS, IF, IHC (p) Wirt: Maus Monoclonal SPM160 unconjugated
Produktnummer ABIN3026680
  • Target Alle Actin, Muscle Specific Produkte
    Actin, Muscle Specific
    Reaktivität
    • 27
    • 26
    • 26
    • 9
    • 9
    • 9
    • 9
    Human, Kaninchen, Ratte
    Wirt
    • 27
    Maus
    Klonalität
    • 27
    Monoklonal
    Konjugat
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    Dieser Actin, Muscle Specific Antikörper ist unkonjugiert
    Applikation
    • 26
    • 26
    • 18
    • 9
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Produktmerkmale
    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    Human myocardium extract was used as the immunogen for this Muscle Specific Actin antibody.
    Klon
    SPM160
    Isotyp
    IgG1 kappa
  • Applikationshinweise
    The optimal dilution of the Muscle Specific Actin antibody for each application should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Konservierungsmittel
    Azide free
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the Muscle Specific Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Actin, Muscle Specific
    Andere Bezeichnung
    Muscle Specific Actin (Actin, Muscle Specific Produkte)
    Hintergrund
    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotype of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
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