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Actin Antikörper

ACTA1 Reaktivität: Human, Maus, Ratte, Kaninchen WB, IHC (p), IF, FACS Wirt: Maus Monoclonal HHF35 unconjugated
Produktnummer ABIN3026689
  • Target Alle Actin (ACTA1) Antikörper anzeigen
    Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
    Reaktivität
    • 88
    • 45
    • 38
    • 15
    • 10
    • 9
    • 9
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Kaninchen
    Wirt
    • 77
    • 30
    Maus
    Klonalität
    • 72
    • 32
    • 1
    Monoklonal
    Konjugat
    • 61
    • 12
    • 11
    • 10
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Actin Antikörper ist unkonjugiert
    Applikation
    • 83
    • 58
    • 42
    • 23
    • 23
    • 22
    • 9
    • 9
    • 6
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)
    Produktmerkmale
    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.
    Klon
    HHF35
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product ACTA1 Primärantikörper
  • Applikationshinweise
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.

    1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL, FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Konservierungsmittel
    Azide free
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
    Andere Bezeichnung
    Muscle Actin (ACTA1 Produkte)
    Hintergrund
    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
    Gen-ID
    58
    Pathways
    Caspase Kaskade in der Apoptose, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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