Actin Antikörper

Produktnummer ABIN3026689

Produktdetails anti-Actin Antikörper

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reaktivität: Human, Maus, Ratte, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Actin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktmerkmale: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Aufreinigung: Protein G affinity chromatography

Immunogen: SDS extract of human myocardium was used as the immunogen for this Muscle actin HHF35 antibody.

Klon: HHF35

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Muscle actin HHF35 antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the IHC staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL, FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Andere Bezeichnung: Muscle Actin (ACTA1 Produkte)

Synonyme: ACTA antikoerper, ASMA antikoerper, CFTD antikoerper, CFTD1 antikoerper, CFTDM antikoerper, MPFD antikoerper, NEM1 antikoerper, NEM2 antikoerper, NEM3 antikoerper, acta antikoerper, asma antikoerper, cftd antikoerper, cftd1 antikoerper, cftdm antikoerper, mpfd antikoerper, nem1 antikoerper, AA959943 antikoerper, Acta-2 antikoerper, Acts antikoerper, Actsk-1 antikoerper, actc antikoerper, actc1 antikoerper, cfk antikoerper, hm:zeh0631 antikoerper, zeh0631 antikoerper, acta1 antikoerper, hm:zeh0293 antikoerper, zgc:112098 antikoerper, actin, alpha 1, skeletal muscle antikoerper, actin, alpha 1, skeletal muscle L homeolog antikoerper, actin, alpha 1b, skeletal muscle antikoerper, actin, muscle antikoerper, actin, alpha skeletal muscle antikoerper, actin, alpha 1a, skeletal muscle antikoerper, ACTA1 antikoerper, acta1.L antikoerper, acta1 antikoerper, Acta1 antikoerper, acta1b antikoerper, LOC100533357 antikoerper, LOC109479779 antikoerper, LOC109108280 antikoerper, acta1a antikoerper

Hintergrund: This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).

Gen-ID: 58

Pathways: Caspase Kaskade in der Apoptose, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development