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KRT1 Antikörper

KRT1 Reaktivität: Human, Ratte, Maus WB, IF, FACS, IHC (p) Wirt: Maus Monoclonal 34BE12 unconjugated
Produktnummer ABIN3026726
  • Target Alle KRT1 Antikörper anzeigen
    KRT1 (Cytokeratin HMW (KRT1))
    Reaktivität
    • 27
    • 27
    • 18
    • 10
    • 10
    • 10
    • 10
    • 9
    • 1
    Human, Ratte, Maus
    Wirt
    • 27
    Maus
    Klonalität
    • 27
    Monoklonal
    Konjugat
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    Dieser KRT1 Antikörper ist unkonjugiert
    Applikation
    • 26
    • 26
    • 17
    • 16
    • 9
    • 9
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Produktmerkmale
    This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    Solubilized keratin extract from human stratum corneum was used as the immunogen for this HMW Cytokeratin antibody.
    Klon
    34BE12
    Isotyp
    IgG1 kappa
  • Applikationshinweise
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HMW Cytokeratin antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells,IF: 0.5-1.0 μg/mL,WB: 0.5-1.0 μg/mL,IHC (FFPE): 0.5-1.0 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Konservierungsmittel
    Azide free
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the HMW Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    KRT1 (Cytokeratin HMW (KRT1))
    Andere Bezeichnung
    HMW Cytokeratin (KRT1 Produkte)
    Synonyme
    CK1 antikoerper, EHK antikoerper, EHK1 antikoerper, EPPK antikoerper, K1 antikoerper, KRT1A antikoerper, NEPPK antikoerper, keratin 1 antikoerper, KRT1 antikoerper
    Hintergrund
    This antibody recognizes HMW Keratin 1, 5, 10 and 14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. The HMW Cytokeratin antibody shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this mAb. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. It has also been useful in separating benign from malignant intraductal breast proliferations.
    Gen-ID
    3848
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