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Neurofilament Antikörper

Reaktivität: Human, Ratte, Schwein, Huhn, Rind (Kuh) FACS, IF, IHC (p) Wirt: Maus Monoclonal NR-4 unconjugated
Produktnummer ABIN3026905
  • Target Alle Neurofilament Produkte
    Neurofilament
    Reaktivität
    • 26
    • 23
    • 21
    • 14
    • 14
    • 2
    Human, Ratte, Schwein, Huhn, Rind (Kuh)
    Wirt
    • 21
    • 7
    • 1
    Maus
    Klonalität
    • 21
    • 8
    Monoklonal
    Konjugat
    • 15
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Neurofilament Antikörper ist unkonjugiert
    Applikation
    • 17
    • 13
    • 9
    • 7
    • 6
    • 5
    • 2
    • 1
    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Produktmerkmale
    This mAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    Crude neurofilament preparation from porcine spinal cord was used as the immunogen for the Neurofilament antibody.
    Klon
    NR-4
    Isotyp
    IgG1
  • Applikationshinweise
    Optimal dilution of the Neurofilament antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 1-2 μg/mL,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the Neurofilament antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Neurofilament
    Abstract
    Neurofilament Produkte
    Hintergrund
    This mAb reacts with a 68  kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68  kDa (NF-L), 160  kDa (NF-M) and 200  kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and cell carcinomas of the lung also express neurofilament.
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