Cullin 4B Antikörper (C-Term)

Produktnummer ABIN3030579

Produktdetails anti-Cullin 4B Antikörper

Target: Cullin 4B (CUL4B)

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cullin 4B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human Cullin 4B (DRDYMERDKENPNQYNYIA) was used as the immunogen for this Cullin 4B antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the Cullin 4B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the Cullin 4B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu Cullin 4B

Target: Cullin 4B (CUL4B)

Andere Bezeichnung: Cullin 4B (CUL4B Produkte)

Synonyme: MRXHF2 antikoerper, MRXS15 antikoerper, MRXSC antikoerper, SFM2 antikoerper, 2700050M05Rik antikoerper, AA409770 antikoerper, CUL-4B antikoerper, mKIAA0695 antikoerper, RGD1564494 antikoerper, im:7140950 antikoerper, im:7150765 antikoerper, MGC115611 antikoerper, Tb03.1J15.620 antikoerper, sfm2 antikoerper, mrxsc antikoerper, mrxhf2 antikoerper, cullin 4B antikoerper, cullin 4B S homeolog antikoerper, CUL4B antikoerper, Cul4b antikoerper, cul4b antikoerper, cul4b.S antikoerper, Tb927.3.1290 antikoerper

Hintergrund: Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING(really interesting new gene) ubiquitin ligase(E3) complex in ubiquitylation. The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain(cullin homology domain) and a series of N-terminal repeats(cullin repeats). Ohtake et al.(2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor(AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B(AhR). Complex assembly and ubiquitin ligase activity of CUL4B(AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B(AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex.

UniProt: Q13620