Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Aufreinigung
Antigen affinity
Immunogen
An amino acid sequence from the C-terminus of human EphA1 (HQKRILCSIQGFKD) was used as the immunogen for this EphA1 antibody (100% homologous in human, mouse and rat).
EPHA1
Reaktivität: Human
WB, IHC, IF, IC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
The stated application concentrations are suggested starting amounts. Titration of the EphA1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Lagerung
-20 °C
Informationen zur Lagerung
After reconstitution, the EphA1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
EPH receptor A1, is a protein that in humans is encoded by the EPHA1 gene. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. The gene is mapped to 7q34-q35. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. They are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene is expressed in some human cancer cell lines and has been implicated in carcinogenesis.