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Myosin Binding Subunit Antikörper (N-Term)

MBS Reaktivität: Human, Maus, Ratte WB, FACS Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3031917
  • Target Alle Myosin Binding Subunit (MBS) Produkte
    Myosin Binding Subunit (MBS)
    Bindungsspezifität
    N-Term
    Reaktivität
    Human, Maus, Ratte
    Wirt
    • 1
    Kaninchen
    Klonalität
    • 1
    Polyklonal
    Konjugat
    • 1
    Dieser Myosin Binding Subunit Antikörper ist unkonjugiert
    Applikation
    Western Blotting (WB), Flow Cytometry (FACS)
    Aufreinigung
    Antigen affinity
    Immunogen
    An amino acid sequence from the N-terminus of human Myosin Phosphatase (MKMADAKQKRNEQLKRW) was used as the immunogen for this Myosin Phosphatase antibody (100% homologous in human, mouse and rat).
    Isotyp
    IgG
  • Applikationshinweise
    The stated application concentrations are suggested starting amounts. Titration of the Myosin Phosphatase antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL, FACS: 1-3 μg/10^6 cells
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Buffer
    0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
    Lagerung
    -20 °C
    Informationen zur Lagerung
    After reconstitution, the Myosin Phosphatase antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
  • Target
    Myosin Binding Subunit (MBS)
    Andere Bezeichnung
    Myosin Phosphatase (MBS Produkte)
    Hintergrund
    PPP1R12A, also called MYPT1 (Myosin phosphatase target subunit 1), is an enzyme that in humans is encoded by the PPP1R12A gene. Sequencing analysis showed that human PPP1R12A contains 1,030 amino acids with a calculated molecular mass of approximately 115 kD. PPP1R12A is the protein that regulates PP1 function in smooth muscle relaxation. The cellular MYPT1-PP1-delta -specific inhibitor CPI17 caused a loss of merlin function characterized by merlin phosphorylation, Ras activation, and transformation. Jin et al. concluded that PPP1R12A and its substrate merlin are part of a previously undescribed tumor suppressor cascade that can be hindered in 2 ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI17.
    UniProt
    O14974
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