NLRP3 Antikörper (N-Term)

Produktnummer ABIN3032021

Produktdetails anti-NLRP3 Antikörper

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Bindungsspezifität: N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NLRP3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of human NLRP3 (RYLEDLEDVDLKKFKMHLED) was used as the immunogen for this NLRP3 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the NLRP3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,IF/ICC: 2-3 μg/mL,FACS: 1-3 μg/10^6 cells

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the NLRP3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Andere Bezeichnung: NLRP3 (NALP3) (NLRP3 Produkte)

Hintergrund: NOD-like receptor family pyrin domain containing 3, also known as CIAS1, CRYOPYRIN, NALP3 or PYPAF1, is a protein that in humans is encoded by the NLRP3 gene. The gene encodes a pyrin-like protein expressed predominantly in peripheral blood leukocytes. NLRP3 interacts with apoptosis-associated speck-like protein containing a CARD(ASC). The encoded protein may play a role in the regulation of inflammation and apoptosis. Mutation of the proteins nucleotide-binding domain reduced ATP binding, Caspase-1 activation, IL-1b production, cell death, macromolecular complex formation, self-association, and association with ASC. Consistent with an essential role for NLRP3 inflammasomes in antifungal immunity, Gross et al.showed that deficient mice are hypersusceptible to C. albicans infection. Activation of the NLRP3 inflammasome in response to virus or to RNA was dependent upon lysosomal maturation and reactive oxygen species production in human cells. The NLRP3 inflammasome senses obesity-associated danger signals and contributes to obesity-induced inflammation and insulin resistance.

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome