Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
Aufreinigung
Antigen affinity
Immunogen
An amino acid sequence from the N-terminus of human NPM1 (ELKADKDYHFKVDNDENEHQ) was used as the immunogen for this Nucleophosmin antibody (100% homologous in human, mouse and rat).
Variations in secondary/substrate sensitivities and test protocols may require the Nucleophosmin antibody to be titrated for optimal performance.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Lagerung
-20 °C
Informationen zur Lagerung
The lyophilized Nucleophosmin antibody can be stored at 4°C to -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaws.
Nucleophosmin, also known as NPM, is a protein that in humans is encoded by the NPM1 gene. The gene maps to chromosome 5q35. Nucleophosmin is likely involved in the assembly of ribosomal proteins into ribosomes. An electron microscopic study indicated that Nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.