Nestin Antikörper (AA 1484-1500)

Produktnummer ABIN303340

Produktdetails anti-Nestin Antikörper

Target: Nestin (NES)

Bindungsspezifität: AA 1484-1500

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Hamster, Kaninchen, Makake

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Nestin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody recognizes Nestin.

Kreuzreaktivität (Details): Species reactivity (expected):Bovine, Dog, Hamster, Macaque, Rabbit, RatSpecies reactivity (tested):Human, Mouse.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Recombinant protein corresponding to amino acids 1484-1500 of Human Nestin.

Anwendungsinformationen

Applikationshinweise: ELISA (1/20000-1/80000). Immunofluorescence. Western Blot (1/500-1/3000). Immunohistochemistry on Paraffin Sections (5 μg/mL).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu Nestin

Target: Nestin (NES)

Andere Bezeichnung: Nestin (NES Produkte)

Hintergrund: Nestin, is a large intermediate filament protein (class Type VI) that was first identified with a monoclonal antibody by Hockfield and McKay (1985). Nestin is expressed predominantly in stem cells of the central nervous system in the neural tube. Nestin is expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker, however, recent studies have found nestin expression in other cell types such as endothelial cells. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.Synonyms: NES, Nbla00170

Gen-ID: 10763

NCBI Accession: NP_006608

UniProt: P48681