Binds to human high molecular weight single-chain urokinase type plasminogen activator (HMW-scuPA, 54 kDa), to high molecular weight two-chain urokinase type plasminogen activator (HMW-tcuPA, 52 kDa) and to low molecular weight single-chain urokinase type plasminogen activator (LMW-scuPA, 33kDa) with high affinity (Kd = 0.1 nM) and does inhibit the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is not inhibited. Highly specific for urokinase, no cross-reactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, and thrombin). Human plasma or plasma proteins do not interfere with its binding to urokinase.
Aufreinigung
Precipitation by PEG-6000 and DEAE-cellulose chromatography (gradient)