VEGFA Antikörper (AA 27-191)

Produktnummer ABIN3042324

Produktdetails anti-VEGFA Antikörper

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Bindungsspezifität: AA 27-191

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VEGFA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Vascular endothelial growth factor A(VEGFA) detection. Tested with WB, IHC-P in Human,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Vascular endothelial growth factor A(VEGFA) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: vascular endothelial growth factor A
Protein Name: Vascular endothelial growth factor A

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human VEGF recombinant protein (Position: A27-R191). Human VEGF shares 78% amino acid (aa) sequence identity with both mouse and rat VEGF.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, The detection limit for VEGF is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-VEGFA Antikörper (ABIN3042324)

Zhang, Chu, Liu, Coates, Shang, Li: "Deer thymosin beta 10 functions as a novel factor for angiogenesis and chondrogenesis during antler growth and regeneration." in: Stem cell research & therapy, Vol. 9, Issue 1, pp. 166, (2019) (PubMed).

Liu, Kuang, Wu, Jin, Sun: "A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo." in: Scientific reports, Vol. 6, pp. 26722, (2018) (PubMed).

Qin, Ke, Zhou, Wang, Liang, Wang, Yang, Gao, Ye, Kumar, Wang: "Metastasis-Associated Protein 1 Deficiency Results in Compromised Pulmonary Alveolar Capillary Angiogenesis in Mice." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 23, pp. 3932-3941, (2018) (PubMed).

Fang, Li, Qiao, Guo, Miao: "Neuroprotective effect of total flavonoids from Ilex pubescens against focal cerebral ischemia/reperfusion injury in rats." in: Molecular medicine reports, Vol. 16, Issue 5, pp. 7439-7449, (2018) (PubMed).

Yang, Zhang, Li, Wen: "MALAT1 enhanced the proliferation of human osteoblasts treated with ultra‑high molecular weight polyethylene by targeting VEGF via miR‑22‑5p." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1536-1546, (2018) (PubMed).

Luo, Zhu, Wang, Yu, Zhou: "MicroRNA-126 affects ovarian cancer cell differentiation and invasion by modulating expression of vascular endothelial growth factor." in: Oncology letters, Vol. 15, Issue 4, pp. 5803-5808, (2018) (PubMed).

Lu, Shi, Ma, Wang, Liu, Chuai, Song, Münsterberg, Cao, Yang: "Proper autophagy is indispensable for angiogenesis during chick embryo development." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 13, pp. 1742-54, (2017) (PubMed).

Gao, Chen, Gao, Wang, Sun, Yang: "CD47 deficiency in tumor stroma promotes tumor progression by enhancing angiogenesis." in: Oncotarget, Vol. 8, Issue 14, pp. 22406-22413, (2017) (PubMed).

Zhang, Yin, Ding, Zhang, Gao: "Vitamin K2 Ameliorates Damage of Blood Vessels by Glucocorticoid: a Potential Mechanism for Its Protective Effects in Glucocorticoid-induced Osteonecrosis of the Femoral Head in a Rat Model." in: International journal of biological sciences, Vol. 12, Issue 7, pp. 776-85, (2017) (PubMed).

Wang, Jiang, Xue: "Recombinant human endostatin reduces hypertrophic scar formation in rabbit ear model through down-regulation of VEGF and TIMP-1." in: African health sciences, Vol. 16, Issue 2, pp. 542-53, (2017) (PubMed).

Huang, Duan, Qian, Fan, Lv, Zhang, Han, Wu, Guo, Hu, Du, Chen, Jin: "Corrigendum: IL-17 Promotes Angiogenic Factors IL-6, IL-8, and Vegf Production via Stat1 in Lung Adenocarcinoma." in: Scientific reports, Vol. 7, pp. 39566, (2017) (PubMed).

Zhang, Liu, Liu, Zhou, Song, Cao, An: "miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression." in: PLoS ONE, Vol. 12, Issue 7, pp. e0179783, (2017) (PubMed).

Dai, Jia, Wang, Yang, Jiang, Chu: "Epidermal growth factor receptor transactivation is involved in the induction of human hepatoma SMMC7721 cell proliferation by insufficient radiofrequency ablation." in: Oncology letters, Vol. 14, Issue 2, pp. 2463-2467, (2017) (PubMed).

Zhang, Li, Zheng, Lin, Zhang, Zhang: "Inhibition of angiogenesis by arsenic trioxide via TSP-1-TGF-β1-CTGF-VEGF functional module in rheumatoid arthritis." in: Oncotarget, Vol. 8, Issue 43, pp. 73529-73546, (2017) (PubMed).

Zhong, Huang, Su, Zhang, Xu, Gong, Chen, Zeng, Chen, Zhou: "Downregulation of miR-199a-5p promotes prostate adeno-carcinoma progression through loss of its inhibition of HIF-1α." in: Oncotarget, Vol. 8, Issue 48, pp. 83523-83538, (2017) (PubMed).

Wang, Wang, Li, Wu, Liu, Yang, Ji, Ji, Li: "The Neuropilin-1 Inhibitor, ATWLPPR Peptide, Prevents Experimental Diabetes-Induced Retinal Injury by Preserving Vascular Integrity and Decreasing Oxidative Stress." in: PLoS ONE, Vol. 10, Issue 11, pp. e0142571, (2016) (PubMed).

Yang, Wang, Guo, Wang: "Correlation and Significance of Urinary Soluble Fas and Vascular Endothelial Growth Factor in Bladder Urothelial Cancer." in: Disease markers, Vol. 2015, pp. 383509, (2016) (PubMed).

Yuan, Christina, Guo, Chu, Liu, Yan: "Involvement of MicroRNA-210 Demethylation in Steroid-associated Osteonecrosis of the Femoral Head." in: Scientific reports, Vol. 6, pp. 20046, (2016) (PubMed).

Zhang, Wang, Dai, Cai, Zhan, Gang, Hu, Xia, Zhang: "Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma." in: Oncotarget, Vol. 7, Issue 7, pp. 8043-54, (2016) (PubMed).

Song, Tao, Wang, Wang, Wei, Shen, Zhou: "Effect of bone mesenchymal stem cells transplantation on the micro-environment of early osteonecrosis of the femoral head." in: International journal of clinical and experimental pathology, Vol. 8, Issue 11, pp. 14528-34, (2016) (PubMed).

Details zu VEGFA

Target: VEGFA (Vascular Endothelial Growth Factor A (VEGFA))

Andere Bezeichnung: VEGFA (VEGFA Produkte)

Synonyme: MVCD1 antikoerper, VEGF antikoerper, VPF antikoerper, Vegf antikoerper, Vegf120 antikoerper, Vegf164 antikoerper, Vegf188 antikoerper, Vpf antikoerper, vegf antikoerper, vegfa antikoerper, wu:fj82c06 antikoerper, VEGF-A antikoerper, VEGF164 antikoerper, eVEGF120 antikoerper, eVEGF164 antikoerper, vegf-a antikoerper, vpf antikoerper, vefg antikoerper, si:dkey-14d8.3 antikoerper, wu:fd42e02 antikoerper, vascular endothelial growth factor A antikoerper, vascular endothelial growth factor Aa antikoerper, vascular endothelial growth factor A L homeolog antikoerper, vascular endothelial growth factor Ab antikoerper, VEGFA antikoerper, Vegfa antikoerper, vegfaa antikoerper, vegfa.L antikoerper, vegfa antikoerper, vegfab antikoerper

Hintergrund: VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is, however, structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. And this gene also can regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What's more, it also stimulates neurogenesis in vitro and in vivo.

Synonyms: MGC70609 antibody|MVCD1 antibody|Vascular endothelial growth factor A antibody|Vascular Endothelial Growth Factor antibody|Vascular Permeability Factor antibody|VEGF-A antibody|VEGFA antibody|VEGFA_HUMAN antibody|VPF antibody

Gen-ID: 7422

UniProt: P15692

Pathways: RTK Signalweg, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling