POLH Antikörper (AA 157-361)

Produktnummer ABIN3042492

Produktdetails anti-POLH Antikörper

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Bindungsspezifität: AA 157-361

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLH Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Rabbit IgG polyclonal antibody for DNA polymerase eta(POLH) detection. Tested with WB in Human,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for DNA polymerase eta(POLH) detection. Tested with WB in Human,Rat.
Gene Name: polymerase (DNA directed), eta
Protein Name: DNA polymerase eta

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human POLH recombinant protein (Position: A157-R361). Human POLH shares 93.6% amino acid (aa) sequence identity with mouse POLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu POLH

Target: POLH (Polymerase (DNA Directed), eta (POLH))

Andere Bezeichnung: POLH (POLH Produkte)

Hintergrund: DNA polymerase eta (Pol η), is a protein that in humans is encoded by the POLH gene. This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA, when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

Synonyms: DNA polymerase eta antibody|FLJ16395 antibody|FLJ21978 antibody|Polh antibody|POLH_HUMAN antibody|polymerase DNA directed eta antibody|RAD30 antibody|RAD30 homolog A antibody|RAD30A antibody|Xeroderma pigmentosum variant type protein antibody|XP V antibody|XPV antibody

Gen-ID: 5429

UniProt: Q9Y253

Pathways: DNA Reparatur