GM-CSF Antikörper (AA 18-141)

Produktnummer ABIN3042527

Produktdetails anti-GM-CSF Antikörper

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Bindungsspezifität: AA 18-141

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GM-CSF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Granulocyte-macrophage colony-stimulating factor(CSF2) detection. Tested with WB, IHC-P in Mouse.

Marke: Picoband™

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Granulocyte-macrophage colony-stimulating factor(CSF2) detection. Tested with WB, IHC-P in Mouse.
Gene Name: colony stimulating factor 2 (granulocyte-macrophage)
Protein Name: Granulocyte-macrophage colony-stimulating factor

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived mouse GM-CSF recombinant protein (Position: A18-K141). Mouse GM-CSF shares 54% and 70% amino acid (aa) sequences identity with human and rat GM-CSF, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, The detection limit for GM-CSF is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-GM-CSF Antikörper (ABIN3042527)

Fang, Shi, Meng, Li, Peng: "The Balance between Conventional DCs and Plasmacytoid DCs Is Pivotal for Immunological Tolerance during Pregnancy in the Mouse." in: Scientific reports, Vol. 6, pp. 26984, (2018) (PubMed).

Details zu GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Andere Bezeichnung: CSF2 (CSF2 Produkte)

Synonyme: GMCSF antikoerper, GM-CSF antikoerper, CSF2 antikoerper, Csfgm antikoerper, Gm-CSf antikoerper, MGI-IGM antikoerper, Gm-csf antikoerper, Gmcsf antikoerper, CSF antikoerper, colony stimulating factor 2 antikoerper, colony stimulating factor 2 (granulocyte-macrophage) antikoerper, CSF2 antikoerper, Csf2 antikoerper

Hintergrund: GM-CSF, Granulocyte-macrophage colony-stimulating factor, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the GM-CSF gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.

Synonyms: Burst Promoting Activity antibody|CMCSF antibody|Colony stimulating factor 2 (granulocyte-macrophage) antibody|Colony Stimulating Factor 2 antibody|Colony stimulating factor antibody|Colony-stimulating factor antibody|CSF 2 antibody|CSF Alpha antibody|CSF antibody|CSF2 antibody|CSF2_HUMAN antibody|Eosinophil Colony Stimulating Factor antibody|GM-CSF antibody|GMCSF antibody|Granulocyte Macrophage Colony Stimulating Factor antibody|Granulocyte-macrophage colony-stimulating factor antibody|MGC131935 antibody|MGC138897 antibody|Molgramostin antibody|Pluripoietin Alpha antibody|Sargramostim antibody

UniProt: P01587

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin