Ubiquitin B Antikörper (N-Term)

Produktnummer ABIN3042699

Produktdetails anti-Ubiquitin B Antikörper

Target: Ubiquitin B (UBB)

Bindungsspezifität: AA 29-45, N-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Ubiquitin B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Polyubiquitin-B(UBB) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: KIQDKEGIPP DQQRLIF

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Polyubiquitin-B(UBB) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: ubiquitin B
Protein Name: Polyubiquitin-B

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Ubiquitin(29-45aa KIQDKEGIPPDQQRLIF), identical to the rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Ubiquitin B

Target: Ubiquitin B (UBB)

Andere Bezeichnung: UBB (UBB Produkte)

Synonyme: AL033289 antikoerper, Rps27a antikoerper, Uba52 antikoerper, Ubb2 antikoerper, Ubc antikoerper, UBC antikoerper, Ubiquitin antikoerper, UBB antikoerper, Ubi-p63E antikoerper, im:6892314 antikoerper, si:ch211-202a12.3 antikoerper, ubc antikoerper, zUBC antikoerper, zgc:172187 antikoerper, ubiquitin B antikoerper, ubiquitin C antikoerper, UBB antikoerper, Ubb antikoerper, UBC antikoerper, ubb antikoerper

Hintergrund: Ubiquitin(originally, ubiquitous immunopoietic polypeptide) is a small regulatory protein that has been found in almost all tissues(ubiquitously) of eukaryotic organisms. Its gene is mapped to 16p 13.3. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Ubiquitin binds to proteins and labels them for destruction. It directs protein recycling and also direct proteins to other locations in the cell, where they control other protein and cell mechanisms.

Synonyms: FLJ25987 antibody|MGC8385 antibody|Polyubiquitin B antibody|RPS 27A antibody|RPS27A antibody|UBA 52 antibody|UBA 80 antibody|UBA52 antibody|UBA80 antibody|UBB antibody|UBB_HUMAN antibody|UBC antibody|UBCEP 1 antibody|UBCEP 2 antibody|UBCEP1 antibody|UBCEP2 antibody|Ubiquitin antibody|ubiquitin B antibody

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, Autophagie, EGFR Downregulation, Ubiquitin Proteasome Pathway