CYP27B1 Antikörper (C-Term)

Produktnummer ABIN3042787

Produktdetails anti-CYP27B1 Antikörper

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Bindungsspezifität: AA 475-508, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CYP27B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (CYP27B1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: HFEVQPEPGA APVRPKTRTV LVPERSINLQ FLDR

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial (CYP27B1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: cytochrome P450, family 27, subfamily B, polypeptide 1
Protein Name: 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CYP27B1 (475-508aa HFEVQPEPGAAPVRPKTRTVLVPERSINLQFLDR), different from the related mouse and rat sequences by six amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu CYP27B1

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Andere Bezeichnung: CYP27B1 (CYP27B1 Produkte)

Synonyme: cp2b antikoerper, cyp1 antikoerper, cyp1alpha antikoerper, cyp27b antikoerper, p450c1 antikoerper, pddr antikoerper, vdd1 antikoerper, vddr antikoerper, vddri antikoerper, vdr antikoerper, CP2B antikoerper, CYP1 antikoerper, CYP1alpha antikoerper, CYP27B antikoerper, P450c1 antikoerper, PDDR antikoerper, VDD1 antikoerper, VDDR antikoerper, VDDRI antikoerper, VDR antikoerper, Cyp40 antikoerper, Cp2b antikoerper, Cyp1 antikoerper, Cyp27b antikoerper, Pddr antikoerper, Vdd1 antikoerper, Vddr antikoerper, VddrI antikoerper, Vdr antikoerper, cytochrome P450 family 27 subfamily B member 1 antikoerper, cytochrome P450, family 27, subfamily B, polypeptide 1 antikoerper, cytochrome P450, family 27, subfamily b, polypeptide 1 antikoerper, cyp27b1 antikoerper, CYP27B1 antikoerper, Cyp27b1 antikoerper

Hintergrund: CYP27B1 belongs to the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the inner mitochondrial membrane where it hydroxylates 25-hydroxyvitamin D3 at the 1alpha position. This reaction synthesizes 1alpha,25-dihydroxyvitamin D3, the active form of vitamin D3, which binds to the vitamin D receptor and regulates calcium metabolism. Thus this enzyme regulates the level of biologically active vitamin D and plays an important role in calcium homeostasis. Mutations in this gene can result in vitamin D-dependent rickets type I.

Synonyms: 1alpha(OH)ase antibody|25-hydroxyvitamin D(3) 1-alpha-hydroxylase antibody|25-hydroxyvitamin D-1 alpha hydroxylase antibody|25-OHD-1 alpha-hydroxylase antibody|Calcidiol 1-monooxygenase antibody|CP27B_HUMAN antibody|CP2B antibody|CYP1 antibody|CYP1ALPHA antibody|CYP27B antibody|Cyp27b1 antibody|Cytochrome p450 27B1 antibody|Cytochrome p450 27B13 antibody|Cytochrome P450 family 27 subfamily B polypeptide 1 antibody|Cytochrome P450 subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase) polypeptide 1 antibody| Cytochrome P450 subfamily XXVIIB polypeptide 1 antibody|Cytochrome P450C1 alpha antibody| Cytochrome P450VD1-alpha antibody|mitochondrial antibody|P450C1 alpha antibody|P450c1 antibody|P450C1-alpha antibody|P450VD1-alpha antibody|PDDR antibody|VD3 1A hydroxylase antibody|VDD1 antibody|VDDR antibody|VDDR I antibody|VDDRI antibody|VDR antibody

Gen-ID: 1594

UniProt: O15528

Pathways: Metabolism of Steroid Hormones and Vitamin D