TNF alpha Antikörper (AA 80-235)

Produktnummer ABIN3042817

Produktdetails anti-TNF alpha Antikörper

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Bindungsspezifität: AA 80-235

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB, IHC-P, ELISA in Mouse.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB, IHC-P, ELISA in Mouse.
Gene Name: tumor necrosis factor
Protein Name: tumor necrosis factor

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived mouse TNF alpha recombinant protein(Position: L80-L235).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Species: Mouse

Notes: Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-TNF alpha Antikörper (ABIN3042817)

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Gu, Ding, Tang, Lei, Jiang, Xu: "A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal Müller Cell Gliosis." in: Frontiers in pharmacology, Vol. 9, pp. 331, (2018) (PubMed).

Wang, Li, Chen, Jiang, Lu, Zhao: "Hydrogen sulfide accelerates wound healing in diabetic rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 5, pp. 5097-104, (2016) (PubMed).

Pan, Wang, Pu, Yao, Wang: "Effect of Puerarin on Expression of ICAM-1 and TNF-α in Kidneys of Diabetic Rats." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 2134-40, (2016) (PubMed).

Chen, Wang, Li, Jiang, Lu, Zhou: "Heme Oxygenase-1 Promotes Delayed Wound Healing in Diabetic Rats." in: Journal of diabetes research, Vol. 2016, pp. 9726503, (2016) (PubMed).

Ke, Li, Chen: "Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1563-1572, (2016) (PubMed).

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Qin, Ma, Yang, Hu, Zhou, Fu, Tian, Liu, Xu, Shen: "A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation and Alleviated Dexamethasone-Induced Insulin Resistance in 3T3-L1 adipocytes." in: Natural products and bioprospecting, Vol. 5, Issue 3, pp. 159-66, (2015) (PubMed).

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Presti, DOrazio, Labra, La Ferla, Mezzasalma, Bizzaro, Giardina, Michelotti, Tursi, Vassallo, Di Gennaro: "Evaluation of the probiotic properties of new Lactobacillus and Bifidobacterium strains and their in vitro effect." in: Applied microbiology and biotechnology, Vol. 99, Issue 13, pp. 5613-26, (2015) (PubMed).

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Xia, Li, Xiang, Chang, Xia, Han: "Neuroprotective effects of an aqueous extract of Futokadsura stem in an A?-induced Alzheimer's disease-like rat model." in: The Chinese journal of physiology, Vol. 58, Issue 2, pp. 104-13, (2015) (PubMed).

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Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: TNF (TNF alpha Produkte)

Synonyme: DIF antikoerper, TNF-alpha antikoerper, TNFA antikoerper, TNFSF2 antikoerper, RATTNF antikoerper, Tnfa antikoerper, tnf antikoerper, TNF-a antikoerper, TNFalpha antikoerper, Tnfsf1a antikoerper, TNFa antikoerper, cTNF antikoerper, Tnf-alpha antikoerper, tnfa-like antikoerper, TNF-ALPHA antikoerper, dif antikoerper, tnfa antikoerper, xtnf antikoerper, tnfsf2 antikoerper, tnf-alpha antikoerper, Cachectin antikoerper, tumor necrosis factor antikoerper, tumor necrosis factor b (TNF superfamily, member 2) antikoerper, tumor necrosis factor alpha antikoerper, tumor necrosis factor a (TNF superfamily, member 2) antikoerper, TNF antikoerper, Tnf antikoerper, tnf antikoerper, tnfb antikoerper, tnf-alpha antikoerper, LOC103694380 antikoerper, tnfa antikoerper

Hintergrund: TNF alpha(Tumor Necrosis Factor alpha) gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor(TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Synonyms: APC1 antibody|APC1 protein antibody|Cachectin antibody|DIF antibody|Differentiation inducing factor antibody|Macrophage cytotoxic factor antibody|MCF antibody|Necrosin antibody|TNF a antibody|TNF alpha antibody|TNF antibody|TNF Macrophage Derived antibody|TNF Monocyte Derived antibody|TNF Superfamily Member 2 antibody|TNF superfamily, member 2 antibody|TNF, macrophage derived antibody|TNF, monocyte derived antibody|TNF-a antibody| TNF-alpha antibody|TNFA antibody|TNFA_HUMAN antibody|TNFSF2 antibody|Tumor necrosis factor alpha antibody|Tumor necrosis factor antibody|Tumor necrosis factor ligand superfamily member 2 antibody|Tumor Necrosis Factor Precursor antibody|Tumor Necrosis Factor, Membrane Form antibody|Tumor necrosis factor, soluble form antibody|Tumour Necrosis Factor Alpha antibody

UniProt: P06804

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome