SPARCL1 Antikörper (C-Term)

Produktnummer ABIN3042909

Produktdetails anti-SPARCL1 Antikörper

Target: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Bindungsspezifität: AA 649-664, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SPARCL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for SPARC-like protein 1(SPARCL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: HCFGIKEEDI DENLLF

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for SPARC-like protein 1(SPARCL1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: SPARC-like 1(hevin)
Protein Name: SPARC-like protein 1

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SPARCL1(649-664aa HCFGIKEEDIDENLLF), identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu SPARCL1

Target: SPARCL1 (SPARC-Like 1 (Hevin) (SPARCL1))

Andere Bezeichnung: SPARCL1 (SPARCL1 Produkte)

Synonyme: LOC445896 antikoerper, SPARCL1 antikoerper, sparcl antikoerper, sparcl1 antikoerper, DKFZp459I2327 antikoerper, Ecm2 antikoerper, Sc1 antikoerper, hevin antikoerper, mast9 antikoerper, PIG33 antikoerper, SC1 antikoerper, SPARCL-1 antikoerper, SPARCL1 antikoerper, SPARC like 1 antikoerper, SPARC-like 1 antikoerper, SPARC-like protein 1 antikoerper, LOC445896 antikoerper, SPARCL1 antikoerper, sparcl1 antikoerper, Sparcl1 antikoerper

Hintergrund: SPARCL1(SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules(HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. Hevin is highly expressed in HEV and is though to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration(Girard and Springer, 1995).

Synonyms: Ecm2 antibody|Extracellular matrix protein 2 antibody|Hevin antibody|High endothelial venule protein antibody|MAST 9 antibody|mast9 antibody|Matrix glycoprotein Sc1 antibody|PIG33 antibody|proliferation inducing protein 33 antibody|RAGS1 antigen antibody|SC1 antibody|SPARC like 1 antibody|SPARC-like protein 1 antibody|Sparcl1 antibody|SPRL1_HUMAN antibody

UniProt: Q14515