RFC1 Antikörper (N-Term)
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- Target Alle RFC1 Antikörper anzeigen
- RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))
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Bindungsspezifität
- AA 46-64, N-Term
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser RFC1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Verwendungszweck
- Rabbit IgG polyclonal antibody for Replication factor C subunit 1(RFC1) detection. Tested with WB, IHC-P in Human.
- Sequenz
- NSSRKEDDFK QKQPSKKKR
- Kreuzreaktivität (Details)
- No cross reactivity with other proteins.
- Produktmerkmale
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Rabbit IgG polyclonal antibody for Replication factor C subunit 1(RFC1) detection. Tested with WB, IHC-P in Human.
Gene Name: replication factor C(activator 1) 1, 145 kDa
Protein Name: Replication factor C subunit 1 - Aufreinigung
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human RFC1(46-64aa NSSRKEDDFKQKQPSKKKR).
- Isotyp
- IgG
- Top Product
- Discover our top product RFC1 Primärantikörper
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- Applikationshinweise
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Kommentare
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Konservierungsmittel
- Thimerosal (Merthiolate), Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Haltbarkeit
- 12 months
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- Target
- RFC1 (Replication Factor C (Activator 1) 1, 145kDa (RFC1))
- Andere Bezeichnung
- RFC1 (RFC1 Produkte)
- Synonyme
- A1 antikoerper, MHCBFB antikoerper, PO-GA antikoerper, RECC1 antikoerper, RFC antikoerper, RFC140 antikoerper, AtRFC1 antikoerper, replication factor C 1 antikoerper, replication factor C1 antikoerper, 140kDa antikoerper, Alp145 antikoerper, Recc1 antikoerper, Replication factor C1 antikoerper, si:dkey-170e5.1 antikoerper, zgc:162875 antikoerper, replication factor C subunit 1 antikoerper, replication factor C1 antikoerper, replication factor C (activator 1) 1 antikoerper, DNA replication factor C complex subunit Rfc1 antikoerper, RFC1 antikoerper, Rfc1 antikoerper, rfc1 antikoerper
- Hintergrund
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RFC(Replication Factor C), also called activator-1, is a multimeric primer-recognition protein consisting of 5 distinct subunits of 145, 40, 38, 37, and 36.5 kD. Human RFC was purified from extracts of HeLa cells as a host factor essential for the in vitro replication of simian virus 40(SV40) DNA. RFC, in the presence of ATP, assembles proliferating-cell nuclear antigen and DNA polymerase-delta or polymerase-epsilon on primed DNA templates. Tight interfacial coordination of the ATP analog ATP-gamma-S by RFC resulted in a spiral arrangement of the ATPase domains of the clamp loader above the PCNA ring.
Synonyms: A1 140 kDa subunit antibody|A1 antibody|A1 P145 Activator 1 large subunit antibody|Activator 1 140 kDa subunit antibody|Activator 1 large subunit antibody|Activator 1 subunit 1 antibody|DNA binding Protein PO GA antibody|DNA-binding protein PO-GA antibody|MHC binding factor beta antibody|MHCBFB antibody|PO GA antibody|RECC1 antibody|Replication factor C(activator 1) 1, 145 kDa antibody|Replication factor C 140 kDa subunit antibody|Replication factor C antibody|Replication factor C large subunit antibody|Replication factor C subunit 1 antibody|Replication factor C1 antibody|RF-C 140 kDa subunit antibody|RFC antibody|RFC1 antibody|RFC1_HUMAN antibody|RFC140 antibody|RFC140 Replication Factor C 140 kDa subunit antibody - UniProt
- P35251
- Pathways
- Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA, Dicarboxylic Acid Transport
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