Sonic Hedgehog Antikörper (N-Term)

Produktnummer ABIN3043230

Produktdetails anti-Sonic Hedgehog Antikörper

Target: Sonic Hedgehog (SHH)

Bindungsspezifität: AA 203-218, N-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Sonic Hedgehog Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Sonic hedgehog protein(SHH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: ATVHLEQGGT KLVKDL

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Sonic hedgehog protein(SHH) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: sonic hedgehog
Protein Name: Sonic hedgehog protein

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Sonic Hedgehog(203-218aa ATVHLEQGGTKLVKDL), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-Sonic Hedgehog Antikörper (ABIN3043230)

Dai, Ai, Du, Chen: "Sonic hedgehog expression correlates with distant metastasis in pancreatic adenocarcinoma." in: Pancreas, Vol. 40, Issue 2, pp. 233-6, (2011) (PubMed).

Details zu Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Andere Bezeichnung: SHH (SHH Produkte)

Synonyme: HHG1 antikoerper, HLP3 antikoerper, HPE3 antikoerper, MCOPCB5 antikoerper, SMMCI antikoerper, TPT antikoerper, TPTPS antikoerper, 9530036O11Rik antikoerper, Dsh antikoerper, Hhg1 antikoerper, Hx antikoerper, Hxl3 antikoerper, M100081 antikoerper, fc83d08 antikoerper, shh antikoerper, syu antikoerper, vhh-1 antikoerper, vhh1 antikoerper, wu:fc83d08 antikoerper, Xhh antikoerper, hedgehog antikoerper, xshh antikoerper, SHH antikoerper, twh antikoerper, twhh antikoerper, sonic hedgehog antikoerper, sonic hedgehog a antikoerper, sonic hedgehog L homeolog antikoerper, sonic hedgehog protein A antikoerper, sonic hedgehog b antikoerper, SHH antikoerper, Shh antikoerper, shha antikoerper, shh.L antikoerper, shh antikoerper, shhb antikoerper

Hintergrund: The mouse, chicken, and zebrafish Shh homologs are highly conserved. SHH expression was not detected in adult tissues examined. However, it was expressed in fetal intestine, liver, lung, and kidney. SHH gene is mapped to 7q. SHH mutations are not a frequent cause of isolated oral clefts.

Synonyms: HHG 1 antibody|HHG-1 antibody|HHG1 antibody|HLP 3 antibody|HLP3 antibody|Holoprosencephaly 3 antibody|HPE 3 antibody|HPE3 antibody|MCOPCB5 antibody|SHH antibody|SHH_HUMAN antibody|SMMC I antibody|SMMCI antibody|Sonic Hedgehog(Drosophila) homolog antibody|sonic hedgehog homolog(Drosophila) antibody|Sonic hedgehog homolog antibody|Sonic hedgehog protein antibody|Sonic hedgehog protein C-product antibody|TPT antibody|TPTPS antibody

UniProt: Q15465

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development