Dihydrofolate Reductase Antikörper (AA 2-187)

Produktnummer ABIN3043286

Produktdetails anti-Dihydrofolate Reductase Antikörper

Target: Dihydrofolate Reductase (DHFR)

Bindungsspezifität: AA 2-187

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Dihydrofolate Reductase Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Dihydrofolate reductase(DHFR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Dihydrofolate reductase(DHFR) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: dihydrofolate reductase
Protein Name: Dihydrofolate reductase

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human DHFR recombinant protein (Position: V2-D187). Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Reacts with: human, mouse, rat

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-Dihydrofolate Reductase Antikörper (ABIN3043286)

Tian, Zhang, Ge, Xiao, Lu, Fu, Liu, Sun: "MicroRNA-205 suppresses proliferation and promotes apoptosis in laryngeal squamous cell carcinoma." in: Medical oncology (Northwood, London, England), Vol. 31, Issue 1, pp. 785, (2013) (PubMed).

Details zu Dihydrofolate Reductase

Target: Dihydrofolate Reductase (DHFR)

Andere Bezeichnung: DHFR (DHFR Produkte)

Hintergrund: Dihydrofolate reductase, or DHFR, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. In humans, the DHFR enzyme is encoded by the DHFR gene. It is found in the q11→q22 region of chromosome 5. What's more, DHFR belongs to the dihydrofolate reductase family, and it converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. DHFR is the key enzyme in folate metabolism. In addition, DHFR catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Synonyms: DHFR antibody|DHFRP1 antibody|Dihydrofolate reductase antibody|DYR antibody|DYR_HUMAN antibody|EC 1.5.1.3 antibody

Gen-ID: 1719

UniProt: P00374

Pathways: Mitotic G1-G1/S Phases