KAP1 Antikörper (AA 699-835)

Produktnummer ABIN3043491

Produktdetails anti-KAP1 Antikörper

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Bindungsspezifität: AA 699-835

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Transcription intermediary factor 1-beta(TRIM28) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Transcription intermediary factor 1-beta(TRIM28) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: tripartite motif containing 28
Protein Name: Transcription intermediary factor 1-beta

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human KAP1 recombinant protein (Position: A699-P835). Human KAP1 shares 94.9% amino acid (aa) sequence identity with both mouse and rat KAP1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Andere Bezeichnung: TRIM28 (TRIM28 Produkte)

Synonyme: KAP1 antikoerper, RNF96 antikoerper, TF1B antikoerper, TIF1B antikoerper, TRIM28 antikoerper, kap1 antikoerper, rnf96 antikoerper, tf1b antikoerper, tif1b antikoerper, AA408787 antikoerper, KAP-1 antikoerper, KRIP-1 antikoerper, Tif1b antikoerper, Tif1beta antikoerper, Kap1 antikoerper, Krip1 antikoerper, TIF1BETA antikoerper, tripartite motif containing 28 antikoerper, tripartite motif containing 28 L homeolog antikoerper, tripartite motif-containing 28 antikoerper, TRIM28 antikoerper, trim28.L antikoerper, Trim28 antikoerper

Hintergrund: Tripartite motif-containing 28 (TRIM28), also known as transcriptional intermediary factor 1β (TIF1β) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.

Synonyms: E3 SUMO protein ligase TRIM28 antibody|E3 SUMO-protein ligase TRIM28 antibody|FLJ29029 antibody|KAP 1 antibody|KAP-1 antibody|KRAB associated protein 1 antibody|KRAB interacting protein 1 antibody|KRAB-associated protein 1 antibody|KRAB-interacting protein 1 antibody|KRIP 1 antibody|KRIP-1 antibody|KRIP1 antibody|Nuclear corepressor KAP 1 antibody|Nuclear corepressor KAP-1 antibody|RING finger protein 96 antibody|RNF96 antibody|TF1B antibody|TIF1 beta antibody|TIF1-beta antibody|TIF1B antibody|TIF1B_HUMAN antibody| Transcription intermediary factor 1 beta antibody|Transcription intermediary factor 1-beta antibody|Trim28 antibody|Tripartite motif containing 28 antibody|tripartite motif containing protein 28 antibody|Tripartite motif-containing protein 28 antibody

Gen-ID: 10155

UniProt: Q13263

Pathways: Hedgehog Signalweg, Positive Regulation of Response to DNA Damage Stimulus