Annexin a1 Antikörper (AA 2-346)

Produktnummer ABIN3043783

Produktdetails anti-Annexin a1 Antikörper

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Bindungsspezifität: AA 2-346

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Annexin a1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Annexin A1(ANXA1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Annexin A1(ANXA1) detection. Tested with WB, IHC-P, IHC-F in Human,Mouse,Rat.
Gene Name: annexin A1
Protein Name: Annexin A1

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Annexin A1 recombinant protein (Position: A2-N346). Human Annexin A1 shares 88% and 89% amino acid (aa) sequences identity with mouse and rat Annexin A1, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Annexin A1 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Mouse
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-Annexin a1 Antikörper (ABIN3043783)

Zhang, Wu, Zha, Zhao, Feng: "The prognostic value and clinicopathological features of sarcomatoid differentiation in patients with renal cell carcinoma: a systematic review and meta-analysis." in: Cancer management and research, Vol. 10, pp. 1687-1703, (2018) (PubMed).

Manville, Abbott: "Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket." in: Nature communications, Vol. 9, Issue 1, pp. 3845, (2018) (PubMed).

Mohamed, Idris, Ahmed, Åstrøm, Mustafa, Ibrahim, Mustafa: "Influence of type 2 diabetes on local production of inflammatory molecules in adults with and without chronic periodontitis: a cross-sectional study." in: BMC oral health, Vol. 15, pp. 86, (2015) (PubMed).

Details zu Annexin a1

Target: Annexin a1 (ANXA1) (Annexin A1 (ANXA1))

Andere Bezeichnung: ANXA1 (ANXA1 Produkte)

Synonyme: anx1a antikoerper, anxa1 antikoerper, wu:fa01d11 antikoerper, wu:fa05d11 antikoerper, wu:fk69h01 antikoerper, MGC64363 antikoerper, anx1 antikoerper, lpc1 antikoerper, MGC89164 antikoerper, ANXA1 antikoerper, ANX1 antikoerper, LPC1 antikoerper, p35 antikoerper, Anx-1 antikoerper, Anx-A1 antikoerper, C430014K04Rik antikoerper, Lpc-1 antikoerper, Lpc1 antikoerper, Anx1 antikoerper, annexin A1a antikoerper, annexin A1 antikoerper, annexin A1 L homeolog antikoerper, Annexin A1 antikoerper, anxa1a antikoerper, anxa1 antikoerper, anxa1.L antikoerper, ANXA1 antikoerper, LOC100335033 antikoerper, Anxa1 antikoerper

Hintergrund: ANXA1, also known as lipocortin I or Annexin A1, is a protein that in humans is encoded by the ANXA1 gene. It is mapped to 9q21.13. ANXA1 belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. ANXA1 protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Lower peptide concentrations possibly found in inflammatory situations elicit Ca(2+) transients without fully activating the mitogen-activated protein kinase pathway. This causes a specific inhibition of the transendothelial migration of neutrophils and a desensitization of neutrophils toward a chemoattractant challenge. These findings identified ANXA1 peptides as novel, endogenous FPR ligands and established a mechanistic basis of ANXA1-mediated antiinflammatory effects.

Synonyms: Annexin 1 antibody|Annexin A1 antibody|Annexin I (lipocortin I) antibody|Annexin I antibody|Annexin-1 antibody|AnnexinA1 antibody|AnnexinI antibody|ANX 1 antibody|ANX A1 antibody|ANX1 antibody|ANXA 1 antibody|ANXA1 antibody|ANXA1 protein antibody|ANXA1_HUMAN antibody|Calpactin 2 antibody|Calpactin II antibody|Calpactin-2 antibody|CalpactinII antibody|Chromobindin 9 antibody|Chromobindin-9 antibody|Chromobindin9 antibody|Lipocortin 1 antibody|Lipocortin I antibody|Lipocortin1 antibody|LipocortinI antibody|LPC 1 antibody|LPC1 antibody|p35 antibody|Phospholipase A2 inhibitory protein antibody

Gen-ID: 301

UniProt: P04083

Pathways: Hormone Transport