HLA-C Antikörper (C-Term)

Produktnummer ABIN3043843

Produktdetails anti-HLA-C Antikörper

Target: HLA-C (Major Histocompatibility Complex Class I C (HLA-C))

Bindungsspezifität: AA 38-62, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HLA-C Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for MHC class I antigen(HLA-C) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequenz: EYWDRETQKY KRQAQADRVN LRKLR

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for MHC class I antigen(HLA-C) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: major histocompatibility complex, class I, C
Protein Name: MHC class I antigen

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human HLA-C(38-62aa EYWDRETQKYKRQAQADRVNLRKLR).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for HLA-C is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu HLA-C

Target: HLA-C (Major Histocompatibility Complex Class I C (HLA-C))

Andere Bezeichnung: HLA-C (HLA-C Produkte)

Synonyme: D6S204 antikoerper, HLA-JY3 antikoerper, HLC-C antikoerper, PSORS1 antikoerper, major histocompatibility complex, class I, C antikoerper, HLA-C antikoerper

Hintergrund: HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Over one hundred HLA-C alleles have been described.

Synonyms: 1C07_HUMAN antibody|Cw-7 alpha chain antibody|D6S204 antibody|HLA class I histocompatibility antigen antibody|HLA class I histocompatibility antigen, C alpha chain antibody|HLA class I histocompatibility antigen, Cw-1 alpha chain antibody|HLA-C antibodyHLA-C histocompatibility type antibody|HLA-Cw antibody|HLA-JY3 antibody|HLAC antibody|HLC-C antibody|human leukocyte antigen-C alpha chain antibody|major histocompatibility antigen HLA-C antibody|major histocompatibility complex, class I, C antibody|MHC class I antigen antibody|MHC class I antigen Cw 1 antibody|MHC class I antigen Cw*7 antibody|MHC class I antigen heavy chain HLA-C antibody|PSORS1 antibody

Gen-ID: 3107

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response