MIF Antikörper (AA 2-115)
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- Target Alle MIF Antikörper anzeigen
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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Bindungsspezifität
- AA 2-115
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser MIF Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Verwendungszweck
- Rabbit IgG polyclonal antibody for Macrophage migration inhibitory factor(MIF) detection. Tested with WB, IHC-P, ICC in Human.
- Kreuzreaktivität (Details)
- No cross reactivity with other proteins.
- Produktmerkmale
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Rabbit IgG polyclonal antibody for Macrophage migration inhibitory factor(MIF) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Protein Name: Macrophage migration inhibitory factor - Aufreinigung
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human MIF recombinant protein (Position: P2-A115). Human MIF shares 89% and 90% amino acid (aa) sequence identity with mouse and rat MIF respectively.
- Isotyp
- IgG
- Top Product
- Discover our top product MIF Primärantikörper
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- Applikationshinweise
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for MIF is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Kommentare
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
- Andere Bezeichnung
- MIF (MIF Produkte)
- Synonyme
- mif antikoerper, Mif antikoerper, gif antikoerper, glif antikoerper, mmif antikoerper, LOC100136498 antikoerper, LOC100284350 antikoerper, LOC100284546 antikoerper, GIF antikoerper, Glif antikoerper, GLIF antikoerper, MMIF antikoerper, macrophage migration inhibitory factor L homeolog antikoerper, macrophage migration inhibitory factor antikoerper, macrophage migration inhibitory factor (glycosylation-inhibiting factor) antikoerper, mif.L antikoerper, mif antikoerper, MIF antikoerper, Mif antikoerper, PHATRDRAFT_49660 antikoerper, LOC100136498 antikoerper, cl405_1 antikoerper, LOC100284546 antikoerper
- Hintergrund
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Macrophage migration inhibitory factor (MIF or MMIF), also known as GIF, is a protein that in humans is encoded by the MIF gene. It is a cytokine released by T-lymphocytes, macrophages, and the pituitary gland that serves to integrate peripheral and central inflammatory responses. MIF gene has 3 exons separated by introns of only 189 and 95 bp, and covers less than 1 kb. The localization of the human gene for MIF is to chromosome 22q11.2. MIF plays a critical role in inflammatory diseases and atherogenesis. It is also involved in cell-mediated immunity and immunoregulation. MIF plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids.
Synonyms: acrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody|GIF antibody|GLIF antibody|Glycosylation inhibiting factor antibody|Glycosylation-inhibiting factor antibody|L-dopachrome isomerase antibody|L-dopachrome tautomerase antibody|Macrophage migration inhibitory factor antibody|Macrophage migration inhibitory factor antibody|MIF antibody|MIF antibody|MIF protein antibody|MIF_HUMAN antibody|MMIF antibody|Phenylpyruvate tautomerase antibody|Phenylpyruvate tautomerase antibody - Gen-ID
- 4282
- UniProt
- P14174
- Pathways
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
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