PKC epsilon Antikörper (AA 53-236)

Produktnummer ABIN3043908

Produktdetails anti-PKC epsilon Antikörper

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Bindungsspezifität: AA 53-236

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKC epsilon Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Protein kinase C epsilon type(PRKCE) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Protein kinase C epsilon type(PRKCE) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: protein kinase C, epsilon
Protein Name: Protein kinase C epsilon type

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human PKC epsilon recombinant protein (Position: Q53-R236). Human PKC epsilon shares 99% amino acid (aa) sequence identity with both mouse and rat PKC epsilon.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for PKC epsilon is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Predicted Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-PKC epsilon Antikörper (ABIN3043908)

Chen, Wang, Zhang, Wan: "Low expression of PKCα and high expression of KRAS predict poor prognosis in patients with colorectal cancer." in: Oncology letters, Vol. 12, Issue 3, pp. 1655-1660, (2016) (PubMed).

Details zu PKC epsilon

Target: PKC epsilon (PRKCE) (Protein Kinase C, epsilon (PRKCE))

Andere Bezeichnung: PRKCE (PRKCE Produkte)

Synonyme: PKCE antikoerper, nPKC-epsilon antikoerper, 5830406C15Rik antikoerper, PKC[e] antikoerper, PKCepsilon antikoerper, Pkce antikoerper, R75156 antikoerper, fj59b07 antikoerper, prkce antikoerper, wu:fj59b07 antikoerper, pkce antikoerper, pkc-epsilon antikoerper, PRKCH antikoerper, protein kinase C epsilon antikoerper, protein kinase C, epsilon antikoerper, protein kinase C, epsilon a antikoerper, PRKCE antikoerper, Prkce antikoerper, prkcea antikoerper, prkce antikoerper

Hintergrund: Protein kinase C epsilon type, also known as PKCE, is an enzyme that in humans is encoded by the PRKCE gene. The protein encoded by this gene is one of the PKC family members. PRKCE is mapped to 2p21. This kinase has been shown to be involved in many different cellular functions, such as apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. It has been found that activation of PRKCE can induce VR1 channel activity at room temperature in the absence of any other agonist. PRKCE gene plays a role in apoptosis signaling pathways in thyroid cells and it has been indicated that a naturally occurring PRKCE mutant that functions as a dominant negative can block cell death triggered by a variety of stimuli. Expression of PRKCE inhibits chemotherapy-induced caspase-3 activation and apoptosis, thereby leading to cell survival.

Synonyms: KPCE_HUMAN antibody|MGC125656 antibody|MGC125657 antibody|nPKC epsilon antibody|nPKC-epsilon antibody|PKCE antibody|Pkcea antibody|PRKCE antibody|Protein kinase C epsilon antibody|Protein kinase C epsilon type antibody

Gen-ID: 5581

UniProt: Q02156

Pathways: T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling