RAG2 Antikörper (C-Term)

Produktnummer ABIN3043918

Produktdetails anti-RAG2 Antikörper

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Bindungsspezifität: AA 498-527, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAG2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for V(D)J recombination-activating protein 2(RAG2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: KKPPMKSLRK KGSGKILTPA KKSFLRRLFD

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for V(D)J recombination-activating protein 2(RAG2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: recombination activating gene 2
Protein Name: V(D)J recombination-activating protein 2

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human RAG2 (498-527aa KKPPMKSLRKKGSGKILTPAKKSFLRRLFD), different from the related mouse sequence by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu RAG2

Target: RAG2 (Recombination Activating Gene 2 (RAG2))

Andere Bezeichnung: RAG2 (RAG2 Produkte)

Synonyme: RAG-2 antikoerper, Rag-2 antikoerper, rag-2 antikoerper, zgc:136743 antikoerper, recombination activating 2 antikoerper, recombination activating gene 2 antikoerper, recombination activating gene 2 S homeolog antikoerper, RAG2 antikoerper, Rag2 antikoerper, rag2 antikoerper, rag2.S antikoerper

Hintergrund: Recombination activating gene 2, also known as RAG-2, is a protein that in humans is encoded by the RAG2 gene. This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms.

Synonyms: RAG 2 antibody|RAG-2 antibody|RAG2 antibody|RAG2_HUMAN antibody|Recombination activating gene 2 antibody|V(D)J recombination activating protein 2 antibody|V(D)J recombination-activating protein 2 antibody

Gen-ID: 5897

UniProt: P55895

Pathways: Chromatin Binding, Production of Molecular Mediator of Immune Response