TFAM Antikörper (N-Term)

Produktnummer ABIN3043945

Produktdetails anti-TFAM Antikörper

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Bindungsspezifität: AA 214-241, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TFAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Transcription factor A, mitochondrial(TFAM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: EMKSWEEQMI EVGRKDLLRR TIKKQRKY

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Transcription factor A, mitochondrial(TFAM) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: transcription factor A, mitochondrial
Protein Name: Transcription factor A, mitochondrial

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA (214-241aa EMKSWEEQMIEVGRKDLLRRTIKKQRKY), different from the related mouse and rat sequences by five amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for mtTFA is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-TFAM Antikörper (ABIN3043945)

Wu, Zhao, Xiao, Peng, Chen, He: "Roles of mitochondrial transcription factor A and microRNA‑590‑3p in the development of colon cancer." in: Molecular medicine reports, Vol. 14, Issue 6, pp. 5475-5480, (2017) (PubMed).

Mo, Peng, Wang, Peng, Lan, Yu: "Roles of mitochondrial transcription factor A and microRNA-590-3p in the development of bladder cancer." in: Oncology letters, Vol. 6, Issue 2, pp. 617-623, (2013) (PubMed).

Details zu TFAM

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Andere Bezeichnung: TFAM (TFAM Produkte)

Synonyme: tcf6 antikoerper, mttf1 antikoerper, mttfa antikoerper, tcf6l1 antikoerper, tcf6l2 antikoerper, tcf6l3 antikoerper, mttfa-A antikoerper, xl-mtTFA antikoerper, MGC153358 antikoerper, zgc:153358 antikoerper, MTTF1 antikoerper, MTTFA antikoerper, TCF6 antikoerper, TCF6L1 antikoerper, TCF6L2 antikoerper, TCF6L3 antikoerper, AI661103 antikoerper, Hmgts antikoerper, mtTFA antikoerper, tsHMG antikoerper, Mttfa antikoerper, transcription factor A, mitochondrial antikoerper, transcription factor A, mitochondrial L homeolog antikoerper, TFAM antikoerper, tfam.L antikoerper, tfam antikoerper, Tfam antikoerper

Hintergrund: TFAM (Transcription factor A, mitochondrial), also known as TCF6 or TCF6L2, is a 162-amino acid protein that activates transcription of each mitochondrial DNA (mtDNA) strand by binding to an element of approximately 30 nucleotides present in both the light-strand and the heavy-strand promoters. By Southern blot analysis of restriction enzyme digests of human/Chinese hamster somatic cell hybrid lines, Milatovich et al. (1992) mapped TFAM sequences, which they called MTTF1, to 3 different chromosomes: chromosomes 10, 7p, and 11q. By PCR-based screening of a somatic cell hybrid panel and by fluorescence in situ hybridization, Scott (2007) stated that the sequences mapped to chromosomes 7p (TCF6L1) and 11q (MTTF1, or TCF6L3) are pseudogenes. Larsson et al. (1997) mapped the mouse mitochondrial transcription factor A gene (Tfam) to the central part of mouse chromosome 10. This region exhibits syntenic homology with human 10q21. Mitochondrial transcription factor A is a key activator of mitochondrial transcription in mammals. It also has a role in mitochondrial DNA replication, since transcription generates an RNA primer necessary for initiation of mtDNA replication.

Synonyms: anscription factor 6-like 1 antibody|Mitochondrial transcription factor 1 antibody| mitochondrial transcription factor A antibody|MtTF1 antibody|mtTFA antibody|TCF 6 antibody| TCF-6 antibody|TCF6 antibody|TCF6L1 antibody|TCF6L2 antibody|TCF6L3 antibody|TFAM antibody| TFAM_HUMAN antibody|Transcription factor 6 antibody|Transcription factor 6 like 2 (mitochondrial transcription factor) antibody|Transcription factor 6 like 2 antibody| Transcription factor 6-like 2 antibody|transcription factor 6-like 3 antibody|Transcription factor A, mitochondrial antibody|Transcription factor A, mitochondrial precursor antibody

Gen-ID: 7019

UniProt: Q00059

Pathways: Chromatin Binding