TLR7 Antikörper (C-Term)

Produktnummer ABIN3043948

Produktdetails anti-TLR7 Antikörper

Target: TLR7 (Toll-Like Receptor 7 (TLR7))

Bindungsspezifität: AA 1020-1047, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TLR7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Toll-like receptor 7(TLR7) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequenz: QAHPYFWQCL KNALATDNHV AYSQVFKE

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Toll-like receptor 7(TLR7) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: toll-like receptor 7
Protein Name: Toll-like receptor 7

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TLR7 (1020-1047aa QAHPYFWQCLKNALATDNHVAYSQVFKE), different from the related mouse sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for TLR7 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Human
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-TLR7 Antikörper (ABIN3043948)

Esancy, Condon, Feng, Kimball, Curtright, Dhaka: "A zebrafish and mouse model for selective pruritus via direct activation of TRPA1." in: eLife, Vol. 7, (2018) (PubMed).

Details zu TLR7

Target: TLR7 (Toll-Like Receptor 7 (TLR7))

Andere Bezeichnung: TLR7 (TLR7 Produkte)

Synonyme: TLR7-like antikoerper, RGD1563357 antikoerper, toll like receptor 7 antikoerper, toll-like receptor 7 antikoerper, TLR7 antikoerper, Tlr7 antikoerper

Hintergrund: TLR7 (Toll-like receptor 7) is protein that in humans is encoded by the TLR7 gene. Orthologs are found in mammals and birds. TLR7 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.

Synonyms: PRO285 antibody|TLR 7 antibody|Tlr7 antibody|TLR7_HUMAN antibody|Toll like receptor 7 antibody|Toll-like receptor 7 antibody|UNQ248 antibody

Gen-ID: 51284

Pathways: TLR Signalweg, Activation of Innate immune Response, Toll-Like Receptors Cascades