Prohibitin 2 Antikörper (Middle Region)

Produktnummer ABIN3044022

Produktdetails anti-Prohibitin 2 Antikörper

Target: Prohibitin 2 (PHB2)

Bindungsspezifität: AA 111-128, Middle Region

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Prohibitin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Verwendungszweck: Rabbit IgG polyclonal antibody for Prohibitin-2(PHB2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequenz: RPNAQELPSM YQRLGLDY

Kreuzreaktivität (Details): Predicted Cross Reactivity: rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Prohibitin-2(PHB2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: prohibitin 2
Protein Name: Prohibitin-2

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human REA(111-128aa RPNAQELPSMYQRLGLDY), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Prohibitin 2

Target: Prohibitin 2 (PHB2)

Andere Bezeichnung: PHB2 (PHB2 Produkte)

Synonyme: bcap37 antikoerper, zgc:73150 antikoerper, wu:fc28c08 antikoerper, PHB2 antikoerper, phb2 antikoerper, ATPHB2 antikoerper, F21M11.21 antikoerper, F21M11_21 antikoerper, prohibitin 2 antikoerper, DKFZp469N1310 antikoerper, BAP antikoerper, BCAP37 antikoerper, Bap37 antikoerper, PNAS-141 antikoerper, REA antikoerper, p22 antikoerper, bap37 antikoerper, prohibitin2 antikoerper, xphb2 antikoerper, AU044498 antikoerper, Bcap37 antikoerper, Bap antikoerper, Bap-37 antikoerper, Bcap27 antikoerper, prohibitin 2a antikoerper, prohibitin 2 antikoerper, prohibitin 2 S homeolog antikoerper, phb2a antikoerper, PHB2 antikoerper, phb2 antikoerper, phb2.S antikoerper, Phb2 antikoerper

Hintergrund: PHB2(Prohibitin 2), also called Repressor of Estrogen Receptor Activity(REA), is a protein that in humans is encoded by the PHB2 gene. The International Radiation Hybrid Mapping Consortium mapped the PHB2 gene to chromosome 12. Montano et al.(1999) showed that REA enhanced the potency of a dominant-negative ER-alpha mutant and antiestrogens as suppressors of ER-alpha activity in Chinese hamster ovary cells. When coexpressed with wildtype ER-alpha or ER-beta(ESR2), REA suppressed activation of a reporter gene in a dose-dependent manner. REA had no effect on reporter activity in the absence of liganded ER, and it had no effect on the transcriptional activities of other hormone receptors. Mutation analysis showed that an N-terminal domain and a central domain of REA were required for its repressor activity.

Synonyms: B cell receptor associated protein BAP37 antibody|B-cell associated protein antibody|B-cell receptor-associated protein BAP37 antibody|Bap 37 antibody|BAP antibody|Bap37 antibody|BCAP 37 antibody|BCAP37 antibody|D prohibitin antibody|D-prohibitin antibody|MGC117268 antibody|p22 antibody|PHB 2 antibody|PHB2 antibody|PHB2_HUMAN antibody|PNAS 141 antibody|PNAS141 antibody|Prohibitin 2 antibody|Prohibitin-2 antibody|Prohibitin2 antibody|Repressor of Estrogen Receptor Activity antibody

UniProt: Q99623

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling