CUEDC2 Antikörper (C-Term)

Produktnummer ABIN3044031

Produktdetails anti-CUEDC2 Antikörper

Target: CUEDC2 (CUE Domain Containing 2 (CUEDC2))

Bindungsspezifität: AA 267-287, C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CUEDC2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for CUE domain-containing protein 2(CUEDC2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: EAEEMKATYI NLKPARKYRF H

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for CUE domain-containing protein 2(CUEDC2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: CUE domain containing 2
Protein Name: CUE domain-containing protein 2

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CUEDC2(267-287aa EAEEMKATYINLKPARKYRFH), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for CUEDC2 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu CUEDC2

Target: CUEDC2 (CUE Domain Containing 2 (CUEDC2))

Andere Bezeichnung: CUEDC2 (CUEDC2 Produkte)

Synonyme: cuedc2 antikoerper, MGC81368 antikoerper, C10orf66 antikoerper, bA18I14.5 antikoerper, 3010002G01Rik antikoerper, wu:fi42e02 antikoerper, zgc:92255 antikoerper, CUE domain containing 2 S homeolog antikoerper, CUE domain containing 2 antikoerper, CUE domain containing 2 L homeolog antikoerper, cuedc2.S antikoerper, CUEDC2 antikoerper, cuedc2.L antikoerper, cuedc2 antikoerper, Cuedc2 antikoerper

Hintergrund: CUEDC2(Cue Domain-Containing Protein 2) is involved in ubiquitin-and proteasome-mediated degradation of progesterone receptor(PR, or PGR) and estrogen receptor(ER)-alpha. Hartz(2011) mapped the CUEDC2 gene to chromosome 10q24.32 based on an alignment of the CUEDC2 sequence with the genomic sequence(GRCh37). Using coimmunoprecipitation analysis and protein pull-down assays, Zhang et al.(2007) confirmed that CUEDC2 interacted with the PRB isoform of PR. Mutation analysis revealed that the IF domain of PRB and the CUE domain of CUEDC2 were required for the interaction. CUEDC2 reduced PRB protein content and promoted progesterone-induced PRB degradation via the ubiquitin-proteasome pathway.

Synonyms: bA18I14.5 antibody|C10orf66 antibody|Chromosome 10 open reading frame 66 antibody|CUE domain containing 2 antibody|CUE domain-containing protein 2 antibody|CUED2 antibody|CUED2_HUMAN antibody|Cuedc2 antibody|MGC2491 antibody|OTTHUMP00000020368 antibody

UniProt: Q9H467