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DDX4 Antikörper (Middle Region)

DDX4 Reaktivität: Human, Maus, Ratte WB, IHC (p), IHC (fro), ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3044032
  • Target Alle DDX4 Antikörper anzeigen
    DDX4 (DEAD Box Protein 4 (DDX4))
    Bindungsspezifität
    • 16
    • 7
    • 7
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 253-272, Middle Region
    Reaktivität
    • 52
    • 23
    • 16
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 65
    • 8
    Kaninchen
    Klonalität
    • 67
    • 6
    Polyklonal
    Konjugat
    • 29
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser DDX4 Antikörper ist unkonjugiert
    Applikation
    • 52
    • 23
    • 16
    • 13
    • 11
    • 10
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
    Verwendungszweck
    Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX4(DDX4) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
    Sequenz
    EDEDSIFAHY QTGINFDKYD
    Kreuzreaktivität (Details)
    No cross reactivity with other proteins.
    Produktmerkmale
    Rabbit IgG polyclonal antibody for Probable ATP-dependent RNA helicase DDX4(DDX4) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
    Gene Name: DEAD(Asp-Glu-Ala-Asp) box polypeptide 4
    Protein Name: Probable ATP-dependent RNA helicase DDX4
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence in the middle region of human DDX4(253-272aa EDEDSIFAHYQTGINFDKYD), identical to the the related rat and mouse sequences.
    Isotyp
    IgG
    Top Product
    Discover our top product DDX4 Primärantikörper
  • Applikationshinweise
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for DDX4 is approximately 0.5 ng/lane under reducing conditions.
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse
    ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Konservierungsmittel
    Thimerosal (Merthiolate), Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    Haltbarkeit
    12 months
  • Target
    DDX4 (DEAD Box Protein 4 (DDX4))
    Andere Bezeichnung
    DDX4 (DDX4 Produkte)
    Synonyme
    VASA antikoerper, AV206478 antikoerper, Mvh antikoerper, RVLG antikoerper, vasa antikoerper, vlg1 antikoerper, DEAD-box helicase 4 antikoerper, DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 antikoerper, DEAD-box helicase 4 L homeolog antikoerper, DDX4 antikoerper, Ddx4 antikoerper, ddx4.L antikoerper
    Hintergrund
    DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.

    Synonyms: DDX 4 antibody|Ddx4 antibody|DDX4_HUMAN antibody|DEAD(Asp Glu Ala Asp) box polypeptide 4 antibody|DEAD box protein 4 antibody|Dead-box protein 4 antibody|DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 4 antibody|MGC111074 antibody|MVH antibody|Probable ATP dependent RNA helicase DDX4 antibody|Probable ATP-dependent RNA helicase DDX4 antibody|VASA antibody|VASA homolog antibody
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