Annexin VII Antikörper (C-Term)

Produktnummer ABIN3044073

Produktdetails anti-Annexin VII Antikörper

Target: Annexin VII (ANXA7) (Annexin A7 (ANXA7))

Bindungsspezifität: AA 445-460, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Annexin VII Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Annexin A7(ANXA7) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: TRSEIDLVQI KQMFAQ

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Annexin A7(ANXA7) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: annexin A7
Protein Name: Annexin A7

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Annexin VII(445-460aa TRSEIDLVQIKQMFAQ), different from the related mouse and rat sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for ANXA7 is approximately 3 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-Annexin VII Antikörper (ABIN3044073)

Zhou, Li, Liu, Li, Wang, Shen: "Increased expression of annexin A7 in temporal lobe tissue of patients with refractory epilepsy." in: Histology and histopathology, Vol. 26, Issue 5, pp. 571-9, (2011) (PubMed).

Details zu Annexin VII

Target: Annexin VII (ANXA7) (Annexin A7 (ANXA7))

Andere Bezeichnung: ANXA7 (ANXA7 Produkte)

Hintergrund: ANXA7(Annexin A7), also known as ANX7 or SNX, is a protein that in humans is encoded by the ANXA7 gene. Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins. Shirvan et al.(1994) isolated genomic clones of the ANXA7 gene and assigned the gene to 10q21.1-q21.2 by study of somatic cell hybrids and by in situ hybridization. Srivastava et al.(2001) concluded that the ANX7 gene exhibits many biologic and genetic properties expected of a tumor suppressor gene and may play a role in prostate cancer progression. Caohuy et al.(1996) present experimental evidence, based on studies of recombinant human ANXA7 and isolated bovine chromaffin cells, that ANXA7 is a Ca(2+)-dependent GTP binding protein. ANXA7 was active in a chromaffin granule aggregation assays in the presence of Ca(2+) and GTP, and was deactivated upon GTP hydrolysis.

Synonyms: Annexin A7 antibody|Annexin VII antibody|Annexin-7 antibody|ANX7 antibody|ANXA7 antibody|ANXA7_HUMAN antibody|SNX antibody|Synexin antibody

UniProt: P20073