DCC Antikörper (N-Term)

Produktnummer ABIN3044119

Produktdetails anti-DCC Antikörper

Target: DCC (Deleted in Colorectal Carcinoma (DCC))

Bindungsspezifität: AA 162-178, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DCC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Netrin receptor DCC(DCC) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: EVIGEPMPTI HWQKNQQ

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Netrin receptor DCC(DCC) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: deleted in colorectal carcinoma
Protein Name: Netrin receptor DCC

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human DCC(162-178aa EVIGEPMPTIHWQKNQQ), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu DCC

Target: DCC (Deleted in Colorectal Carcinoma (DCC))

Andere Bezeichnung: DCC (DCC Produkte)

Synonyme: CRC18 antikoerper, CRCR1 antikoerper, IGDCC1 antikoerper, MRMV1 antikoerper, DCC antikoerper, zdcc antikoerper, C030036D22Rik antikoerper, Igdcc1 antikoerper, xdcc antikoerper, XDCCa antikoerper, dcca-A antikoerper, Dcc antikoerper, DCC netrin 1 receptor antikoerper, deleted in colorectal carcinoma antikoerper, netrin receptor DCC antikoerper, DCC antikoerper, dcc antikoerper, Dcc antikoerper, LOC100712666 antikoerper

Hintergrund: DCC(Deleted IN Colorectal Carcinoma) also known as CRC18 or CRCR1. The DCC gene encodes a functional receptor for netrin and mediates axon outgrowth and the steering response. Heterozygous loss-of-function mutations in DCC can result in congenital mirror movements. Alterations in DCC occur frequently in colorectal cancer. Studying a YAC contig containing the entire DCC coding region, they showed that the DCC gene spans approximately 1.4 Mb. Vogelstein(1995) stated that the precise location of the DCC gene was though to be 18q21.3. DCC is a receptor or a component of a receptor that mediates the effects of netrin-1 on commissural axons, and they complement genetic evidence for interactions between DCC and netrin homologs in C. DCC protein could be detected in varying abundance in all specimens of normal colonic mucosa analyzed as well as in all specimens of adenomatous polyps, colorectal carcinoma and colorectal liver metastases. DCC may function as a tumor-suppressor protein by inducing apoptosis in settings in which ligand is unavailable(for example, during metastasis or tumor growth beyond local blood supply) through functional caspase cascades by a mechanism that requires cleavage of DCC at asp1290. Stein et al.(2001) concluded that DCC plays a central role in netrin signaling of axon growth and guidance independent of A2B receptor activation.

Synonyms: Colorectal cancer related chromosome sequence 18 antibody|Colorectal cancer suppressor antibody|CRC 18 antibody|CRC18 antibody|CRCR 1 antibody|CRCR1 antibody|DCC antibody|DCC_HUMAN antibody|Deleted in colorectal cancer protein antibody|Deleted in colorectal carcinoma antibody|Immunoglobulin superfamily DCC subclass member 1 antibody|Netrin receptor DCC antibody|Tumor suppressor protein DCC antibody

UniProt: P43146

Pathways: Regulation of Cell Size