HSPE1 Antikörper (C-Term)

Produktnummer ABIN3044176

Produktdetails anti-HSPE1 Antikörper

Target: HSPE1 (Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1))

Bindungsspezifität: AA 81-102, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSPE1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for 10 kDa heat shock protein, mitochondrial(HSPE1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequenz: VVLDDKDYFL FRDGDILGKY VD

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for 10 kDa heat shock protein, mitochondrial(HSPE1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: heat shock 10 kDa protein 1(chaperonin 10)
Protein Name: 10 kDa heat shock protein, mitochondrial(Hsp10)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cpn10(81-102aa VVLDDKDYFLFRDGDILGKYVD), different from the related rat and mouse sequences by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu HSPE1

Target: HSPE1 (Heat Shock 10kDa Protein 1 (Chaperonin 10) (HSPE1))

Andere Bezeichnung: HSPE1 (HSPE1 Produkte)

Synonyme: CPN10 antikoerper, EPF antikoerper, GROES antikoerper, HSP10 antikoerper, groS antikoerper, Cpn10 antikoerper, cpn10 antikoerper, zgc:86747 antikoerper, hspe1 antikoerper, MGC89647 antikoerper, HSPE1 antikoerper, MITOCHONDRIAL CHAPERONIN 10 antikoerper, T15D22.2 antikoerper, T15D22_2 antikoerper, chaperonin 10 antikoerper, Dmel\\CG11267 antikoerper, Hsp10 antikoerper, anon-WO0118547.175 antikoerper, hsp10 antikoerper, crpB antikoerper, chpS antikoerper, 10kDa antikoerper, mt-cpn10 antikoerper, heat shock protein family E (Hsp10) member 1 antikoerper, molecular chaperone GroES antikoerper, chaperonin 10 kDa antikoerper, chaperonin, 10 kDa antikoerper, chaperonin-10 kDa antikoerper, heat shock protein family E member 1 antikoerper, heat shock 10 protein 1 antikoerper, heat shock protein family E (Hsp10) member 1 L homeolog antikoerper, heat shock 10kDa protein 1 (chaperonin 10) pseudogene antikoerper, 10 kd chaperonin antikoerper, chaperonin 10 antikoerper, Hsp10p antikoerper, co-chaperonin 10, mitochondrial antikoerper, CG11267 gene product from transcript CG11267-RA antikoerper, co-chaperonin GroES antikoerper, mitochondrial heat shock protein Hsp10 (predicted) antikoerper, chaperonin GroS antikoerper, Hsp10 10 kDa chaperonin GROES antikoerper, mitochondrial heat shock protein Hsp10 antikoerper, heat shock protein 10 antikoerper, heat shock protein 1 (chaperonin 10) antikoerper, heat shock 10kDa protein 1 (chaperonin 10) antikoerper, HSPE1 antikoerper, groES antikoerper, groES2 antikoerper, TP01_0190 antikoerper, Cag_1167 antikoerper, Bm1_56470 antikoerper, Hspe1 antikoerper, hspe1 antikoerper, hspe1.L antikoerper, LOC452179 antikoerper, Cpn10 antikoerper, CPN10 antikoerper, HSP10 antikoerper, CG11267 antikoerper, hsp10 antikoerper, groS antikoerper

Hintergrund: HSPE1(heat shock 10 kDa protein 1(chaperonin 10)), also called CPN10, GROES, CHAPERONIN 10 HOMOLOG, cpn10 HOMOLOG or HSP10, is a protein that in humans is encoded by the HSPE1 gene. GroES is a heptameric ring of identical 10.4-kD subunits that binds to each end of GroEL to form a symmetric, functional heterodimer. The HSP10 gene consists of 4 exons. The HSPE1 gene is mapped to 2q33.1. The transcriptional activity of the promoter fragment in the HSP60 direction is approximately twice that in the HSP10 direction under normal growth conditions, upon heat shock, promoter activity in either direction increased by a factor of approximately 12. Mutational drifts performed in vitro with 4 different enzymes indicated the GroES overexpression doubled the number of accumulating mutations, and promoted the folding of enzyme variants carrying mutations in the protein core and/or mutations with higher destabilizing effects.

Synonyms: 10 kDa chaperonin antibody|10 kDa heat shock protein mitochondrial antibody|10 kDa heat shock protein, mitochondrial antibody|CH10_HUMAN antibody|Chaperonin 10 antibody|Cpn 10 antibody|CPN10 antibody|Early pregnancy factor antibody|Early-pregnancy factor antibody|EPF antibody|GROES antibody|Heat shock 10kD protein 1 antibody|Heat shock 10kD protein 1 chaperonin 10 antibody|Heat shock 10 kDa protein 1 antibody|Heat shock 10 kDa protein 1 chaperonin 10 antibody|HSP 10 antibody|HSP10 antibody|HSPE 1 antibody|HSPE1 antibody

UniProt: P61604

Pathways: Positive Regulation of Endopeptidase Activity