LASP1 Antikörper (N-Term)

Produktnummer ABIN3044177

Produktdetails anti-LASP1 Antikörper

Target: LASP1 (LIM and SH3 Protein 1 (LASP1))

Bindungsspezifität: AA 16-30, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LASP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Verwendungszweck: Rabbit IgG polyclonal antibody for LIM and SH3 domain protein 1(LASP1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequenz: EKVNCLDKFW HKACF

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for LIM and SH3 domain protein 1(LASP1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: LIM and SH3 protein 1
Protein Name: LIM and SH3 domain protein 1(LASP-1)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human LASP1(16-30aa EKVNCLDKFWHKACF), identical to the related rat sequence and different from the related mouse sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu LASP1

Target: LASP1 (LIM and SH3 Protein 1 (LASP1))

Andere Bezeichnung: LASP1 (LASP1 Produkte)

Synonyme: lasp1 antikoerper, MGC53336 antikoerper, LASP1 antikoerper, MGC89667 antikoerper, Lasp-1 antikoerper, MLN50 antikoerper, AA408629 antikoerper, Def-4 antikoerper, SH3P6 antikoerper, Tg(Col1a1-lacZ)1Ngma antikoerper, fb92f05 antikoerper, wu:fb92f05 antikoerper, zgc:77542 antikoerper, lasp-1 antikoerper, mln50 antikoerper, LIM and SH3 protein 1 antikoerper, LIM and SH3 protein 1 L homeolog antikoerper, lasp1 antikoerper, lasp1.L antikoerper, LASP1 antikoerper, Lasp1 antikoerper

Hintergrund: LASP1(LIM and SH3 domain protein 1), also known as LASP1, is a protein that in humans is encoded by the LASP1 gene. This gene encodes a member of a LIM protein subfamily which is characterized by a LIM motif and a domain of Src homology region 3. This protein functions as an actin-binding protein and possibly in cytoskeletal organization. LASP1 has been shown to interact with Zyxin. Tomasetto et al.(1995) mapped the LASP1 gene to chromosome 17q11-q21.3 by radioactive in situ hybridization. Northern blot analysis revealed that LASP1 mRNA was expressed at a basal level in all normal tissues examined and overexpressed in 8 % of primary breast cancers. In most of these cancers, LASP1 and ERBB2 were simultaneously overexpressed.

Synonyms: LASP 1 antibody|LASP-1 antibody|LASP1 antibody|LASP1_HUMAN antibody|LIM and SH3 domain protein 1 antibody|LIM and SH3 protein 1 antibody|Metastatic lymph node gene 50 protein antibody|MLN 50 antibody|MLN50 antibody|OTTHUMP00000164238 antibody|OTTHUMP00000164239 antibody

UniProt: Q14847