HSPB2 Antikörper (Middle Region)

Produktnummer ABIN3044185

Produktdetails anti-HSPB2 Antikörper

Target: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Bindungsspezifität: AA 102-118, Middle Region

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSPB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for Heat shock protein beta-2(HSPB2) detection. Tested with WB, IHC-F, ICC in Human,Mouse,Rat.

Sequenz: RHPQRLDRHG FVSREFC

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Heat shock protein beta-2(HSPB2) detection. Tested with WB, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: heat shock 27 kDa protein 2(HspB2)
Protein Name: Heat shock protein beta-2(HspB2)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HSPB2(102-118aa RHPQRLDRHGFVSREFC), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu HSPB2

Target: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Andere Bezeichnung: HSPB2 (HSPB2 Produkte)

Synonyme: zgc:112532 antikoerper, HSP27 antikoerper, Hs.78846 antikoerper, LOH11CR1K antikoerper, MKBP antikoerper, 27kDa antikoerper, 2810021G24Rik antikoerper, heat shock protein family B (small) member 2 antikoerper, heat shock protein, alpha-crystallin-related, b2 antikoerper, heat shock protein 2 antikoerper, HSPB2 antikoerper, hspb2 antikoerper, Hspb2 antikoerper

Hintergrund: Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein(HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al.(1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al.(1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYAB gene is highly expressed in all 3 tissues. The 2 related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.

Synonyms: DMPK-binding protein antibody|Heat shock 27kD protein 2 antibody|Heat shock 27 kDa protein 2 antibody|Heat shock protein beta-2 antibody|Heat-shock protein beta-2 antibody|Hs.78846 antibody|HSP27 antibody|HspB2 antibody|HSPB2_HUMAN antibody|LOH11CR1K antibody|MGC133245 antibody|MKBP antibody

UniProt: Q16082