NF-kB p65 Antikörper (Middle Region)

Produktnummer ABIN3044253

Produktdetails anti-NF-kB p65 Antikörper

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Bindungsspezifität: AA 143-158, Middle Region

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NF-kB p65 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Verwendungszweck: Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequenz: VPIEEQRGDY DLNAVR

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Transcription factor p65(RELA) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: v-rel reticuloendotheliosis viral oncogene homolog A(avian)
Protein Name: Transcription factor p65

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human NF-kB p65(143-158aa VPIEEQRGDYDLNAVR), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-NF-kB p65 Antikörper (ABIN3044253)

Hu, Fan, Li, Guan, Qu, Pei, Liu: "Bortezomib protects against dextran sulfate sodium‑induced ulcerative colitis in mice." in: Molecular medicine reports, Vol. 15, Issue 6, pp. 4093-4099, (2018) (PubMed).

"Retraction: Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction." in: PLoS ONE, Vol. 13, Issue 2, pp. e0192465, (2018) (PubMed).

Zhou, Li, Shi, Mao, Liu, Chang, Gan, Kuang, Du: "Protective Effect of Klotho against Ischemic Brain Injury Is Associated with Inhibition of RIG-I/NF-κB Signaling." in: Frontiers in pharmacology, Vol. 8, pp. 950, (2018) (PubMed).

Liu, Zhang, Han, Wang, Liu, Zhang, Zhou, Xiang: "[Corrigendum] Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." in: Molecular medicine reports, Vol. 17, Issue 5, pp. 6926, (2018) (PubMed).

Wang, Liu, Wang, Wang, Tang, Jiang: "Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-κB Signaling." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 1282-1294, (2018) (PubMed).

Liu, Bao, Zeng, Wei: "Effects of resveratrol and genistein on nuclear factor‑κB, tumor necrosis factor‑α and matrix metalloproteinase‑9 in patients with chronic obstructive pulmonary disease." in: Molecular medicine reports, Vol. 13, Issue 5, pp. 4266-72, (2017) (PubMed).

Yu, Wang, He, Wang, Gao, Ren, Shi et al.: "Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling ..." in: Cell stress & chaperones, Vol. 21, Issue 4, pp. 697-705, (2017) (PubMed).

Wu, Gao, Li, Yao, Wang, Chen, Li, Ma, Ji, Li: "Hydrogen Sulfide Mitigates Kidney Injury in High Fat Diet-Induced Obese Mice." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 2715718, (2017) (PubMed).

Xie, Zhong, Li: "Comment on "Puerarin Improves Diabetic Aorta Injury by Inhibiting NADPH Oxidase-Derived Oxidative Stress in STZ-Induced Diabetic Rats"." in: Journal of diabetes research, Vol. 2016, pp. 7302620, (2017) (PubMed).

Sun, Yang, Zhang, Zhao: "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." in: Experimental animals, (2017) (PubMed).

Yang, Gao, Wu, Yu, Li, Meng, Li, Yan, Jin: "Epigallocatechin-3-gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression." in: Experimental and therapeutic medicine, Vol. 14, Issue 3, pp. 1975-1982, (2017) (PubMed).

Zhao, Ding, Liu, Yin, Zhang, Ma: "Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damage in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 14, Issue 6, pp. 5515-5522, (2017) (PubMed).

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Huang, Zhong, Zhang, Wang, Xu, Zhu, Wang, Hu, Zhao: "Down-regulation of HMGB1 expression by shRNA constructs inhibits the bioactivity of urothelial carcinoma cell lines via the NF-κB pathway." in: Scientific reports, Vol. 5, pp. 12807, (2016) (PubMed).

Tang, Gao, Mao, He, Liu, Cai, Lin, Wu: "Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathways." in: Cell stress & chaperones, Vol. 21, Issue 2, pp. 239-49, (2016) (PubMed).

Zuo, Zheng, Liu, Ma: "Fenofibrate, a PPARα agonist, protect proximal tubular cells from albumin-bound fatty acids induced apoptosis via the activation of NF-kB." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 10653-61, (2016) (PubMed).

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Details zu NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Andere Bezeichnung: RELA (NFkBP65 Produkte)

Synonyme: C-Rel antikoerper, NFKB3 antikoerper, p65 antikoerper, NFkB antikoerper, c-rel antikoerper, zgc:100833 antikoerper, Xrel2 antikoerper, Xrel3 antikoerper, rel antikoerper, rel-A antikoerper, rel2 antikoerper, rel3 antikoerper, v-rel antikoerper, xrel antikoerper, REL proto-oncogene, NF-kB subunit antikoerper, RELA proto-oncogene, NF-kB subunit antikoerper, v-rel reticuloendotheliosis viral oncogene homolog A (avian) antikoerper, v-rel avian reticuloendotheliosis viral oncogene homolog antikoerper, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog antikoerper, REL antikoerper, RELA antikoerper, Rela antikoerper, Rel antikoerper, rel antikoerper, rel.L antikoerper

Hintergrund: RELA(V-REL AVIAN RETICULOENDOTHELIOSIS VIRAL ONCOGENE HOMOLOG A), also called NFKB3 or NFKB, p65 SUBUNIT. NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins, which inactivate NFKB by trapping it in the cytoplasm. The p65(RELA) heterodimer is the most abundant form of NFKB. And the RELA gene is located on 11q13.1. RELA is a nonhistone substrate of HDAC3 and that IKBA-dependent nuclear export of the HDAC3-deacetylated RELA replenishes the depleted cytoplasmic pool of latent NFKB-IKBA complexes for subsequent NFKB responses. RELA nucleocytoplasmic redistribution coincided with export of PPARG, and immunoprecipitation analysis indicated that PPARG-RELA association was dependent on the PPARG C-terminal ligand-binding domain. IKK-dependent phosphorylation of RELA on ser468 enhanced binding of GCN5 to RELA and RELA ubiquitination.

Synonyms: Avian reticuloendotheliosis viral(v rel) oncogene homolog A antibody|MGC131774 antibody|NF kappa B p65delta3 antibody|NFKB 3 antibody|NFKB3 antibody|Nuclear Factor NF Kappa B p65 Subunit antibody|Nuclear factor NF-kappa-B p65 subunit antibody|Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody|Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody|Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody|OTTHUMP00000233473 antibody|OTTHUMP00000233474 antibody|OTTHUMP00000233475 antibody|OTTHUMP00000233476 antibody|OTTHUMP00000233900 antibody|p65 antibody|p65 NF kappaB antibody|p65 NFkB antibody|RELA antibody|TF65_HUMAN antibody|Transcription Factor p65 antibody|v rel avian reticuloendotheliosis viral oncogene homolog A(nuclear factor of kappa light polypeptide gene enhancer in B cells 3(p65)) antibody|V Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A antibody|v rel reticuloendotheliosis viral oncogene homolog A(avian) antibody|V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody|v-rel reticuloendotheliosis viral oncogene homolog A antibody

UniProt: Q04206

Pathways: NF-kappaB Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, TLR Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteine