BRCA1 Antikörper (AA 1661-1863)

Produktnummer ABIN3044313

Produktdetails anti-BRCA1 Antikörper

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Bindungsspezifität: AA 1661-1863

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRCA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Breast cancer type 1 susceptibility protein(BRCA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Marke: Picoband™

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Breast cancer type 1 susceptibility protein(BRCA1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: breast cancer 1, early onset
Protein Name: Breast cancer type 1 susceptibility protein

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human BRCA1 recombinant protein (Position: E1661-Y1863). Human BRCA1 shares 65% and 66% amino acid (aa) sequence identity with mouse and rat BRCA1, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-BRCA1 Antikörper (ABIN3044313)

Liu, Lai, Li, Sun, Cheng, Ge, Wang, Li, Zhang, De Felici, Dyce, Shen: "Di (2-ethylhexyl) phthalate exposure impairs meiotic progression and DNA damage repair in fetal mouse oocytes in vitro." in: Cell death & disease, Vol. 8, Issue 8, pp. e2966, (2017) (PubMed).

Zhang, Zhang, Cong, Zhang: "The ectopic expression of BRCA1 is associated with genesis, progression, and prognosis of breast cancer in young patients." in: Diagnostic pathology, Vol. 7, pp. 181, (2013) (PubMed).

Details zu BRCA1

Target: BRCA1 (Breast Cancer 1 (BRCA1))

Andere Bezeichnung: BRCA1 (BRCA1 Produkte)

Synonyme: BRCAI antikoerper, BRCC1 antikoerper, BROVCA1 antikoerper, IRIS antikoerper, PNCA4 antikoerper, PPP1R53 antikoerper, PSCP antikoerper, RNF53 antikoerper, iris antikoerper, pscp antikoerper, brcai antikoerper, brcc1 antikoerper, rnf53 antikoerper, brca1 antikoerper, BRCA1 antikoerper, LOC100224649 antikoerper, BRCA1, DNA repair associated antikoerper, breast cancer 1 antikoerper, breast cancer 1, early onset antikoerper, breast cancer 1 L homeolog antikoerper, BRCA1 antikoerper, brca1 antikoerper, LOC755086 antikoerper, Brca1 antikoerper, brca1.L antikoerper

Hintergrund: BRCA1, also known as BRCC1, is a gene which mapping to 17q21.3. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by direct mutation or by alterations in gene expression.

Synonyms: BRAC 1 antibody|BRCA 1 antibody|BRCA1 antibody|BRCA1/BRCA2 containing complex subunit 1 antibody|BRCA1/BRCA2-containing complex, subunit 1 antibody|BRCA1_HUMAN antibody|BRCAI antibody|BRCC 1 antibody|BRCC1 antibody|Breast and ovarian cancer susceptibility protein 1 antibody|Breast Cancer 1 antibody|Breast Cancer 1 Early Onset antibody|Breast cancer type 1 susceptibility protein antibody|Breast Ovarian Cancer Susceptibility antibody|Breast Ovarian Cancer Susceptibility antibody|BROVCA1 antibody|IRIS antibody|Papillary Serous Carcinoma Of The Peritoneum antibody|PNCA4 antibody|PPP1R53 antibody|Protein phosphatase 1 regulatory subunit 53 antibody|PSCP antibody|RING finger protein 53 antibody|RING finger protein 53 antibody|RNF53 antibody

Gen-ID: 672

UniProt: P38398

Pathways: Zellzyklus, DNA Reparatur, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus