PMVK Antikörper (Middle Region)

Produktnummer ABIN3044328

Produktdetails anti-PMVK Antikörper

Target: PMVK (Phosphomevalonate Kinase (PMVK))

Bindungsspezifität: AA 135-151, Middle Region

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PMVK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Phosphomevalonate kinase(PMVK) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: EQSRQQRGWV FTPGVDD

Kreuzreaktivität (Details): Predicted Cross Reactivity: human, mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Phosphomevalonate kinase(PMVK) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: phosphomevalonate kinase
Protein Name: Phosphomevalonate kinase(PMKase/hPMK)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PMVK(135-151aa EQSRQQRGWVFTPGVDD), identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu PMVK

Target: PMVK (Phosphomevalonate Kinase (PMVK))

Andere Bezeichnung: PMVK (PMVK Produkte)

Synonyme: MPK_Bm antikoerper, F5M6.9 antikoerper, F5M6_9 antikoerper, zgc:162701 antikoerper, HUMPMKI antikoerper, PMK antikoerper, PMKA antikoerper, PMKASE antikoerper, 1110011E12Rik antikoerper, 2900002L22Rik antikoerper, AV001327 antikoerper, phosphomevalonate kinase antikoerper, GHMP kinase family protein antikoerper, PMVK antikoerper, Mpk antikoerper, AT1G31910 antikoerper, CpipJ_CPIJ015413 antikoerper, Bm1_05275 antikoerper, pmvk antikoerper, LOAG_11811 antikoerper, Tsp_06629 antikoerper, LOC100282968 antikoerper, Pmvk antikoerper

Hintergrund: PMVK(phosphomevalonate kinase) is a cytosolic enzyme that catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate. It is mapped to chromosome 1p13-1q22-23 and spans more than 8.4 kb in the human genome. PMVK is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. And PMKase gene expression is subject to regulation by sterol at the level of transcription. It is a single copy gene covering less than 15 kb in the human genome. The human PMKase amino acid sequence contains a consensus peroxisomal targeting sequence(PTS-1), Ser-Arg-Leu, at the C terminus of the protein.

Synonyms: hPMK antibody|HUMPMKI antibody|OTTHUMP00000035546 antibody|Phosphomevalonate kinase antibody|PMK antibody|PMK PEN antibody|PMKA antibody|PMKase antibody|PMKI antibody|PMKPEN antibody|PMVK antibody|PMVK_HUMAN antibody

UniProt: Q15126