S100B Antikörper (C-Term)

Produktnummer ABIN3044359

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch S100B in WB. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus und wurde in 17+ Publikationen erwähnt.

Kurzübersicht für S100B Antikörper (C-Term) (ABIN3044359)

Target: S100B (S100 Calcium Binding Protein B (S100B))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser S100B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-S100B Antikörper

Bindungsspezifität: AA 64-78, C-Term

Verwendungszweck: Anti-S100 beta/S100B Antibody Picoband®

Sequenz: DGDGECDFQE FMAFV

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti- beta/B Antibody (ABIN3044359). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human S100 beta, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Wainwright, M. S., Craft, J. M., Griffin, W. S. T., Marks, A., Pineda, J., Padgett, K. R., Van Eldik, L. J. : Increased susceptibility of S100B transgenic mice to perinatal hypoxia-ischemia. Ann. Neurol. 56: 61-67, 2004. 2. Reeves, R. H., Yao, J., Crowley, M. R., Buck, S., Zhang, X., Yarowsky, P., Gearhart, J. D., Hilt, D. C. : Astrocytosis and axonal proliferation in the hippocampus of S100b transgenic mice. Proc. Nat. Acad. Sci. 91: 5359-5363, 1994.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-S100B Antikörper (ABIN3044359)

Liu, Lv, Zhu, Wu, Zhang, Xu, Zheng, Zhao: "Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo." in: Scientific reports, Vol. 7, pp. 39869, (2018) (PubMed).

Sun, Li, Ma, Feng, Song, Lv, He: "Inhibition of HMGB1 reduces rat spinal cord astrocytic swelling and AQP4 expression after oxygen-glucose deprivation and reoxygenation via TLR4 and NF-κB signaling in an IL-6-dependent manner." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 231, (2018) (PubMed).

Xu, Wu, Zhang, Lv, Zheng, Zhao: "Pro‑neurogenic effects of andrographolide on RSC96 Schwann cells in vitro." in: Molecular medicine reports, Vol. 14, Issue 4, pp. 3573-80, (2017) (PubMed).

Zuo, Wu, Zhang, Lv, Xu, Jiang, Zheng, Zhao: "Baicalin promotes the viability of Schwann cells in vitro by regulating neurotrophic factors." in: Experimental and therapeutic medicine, Vol. 14, Issue 1, pp. 507-514, (2017) (PubMed).

Wang, Tian, Xiao, Zhang, Sun, Tang: "A metanephric adenoma of the kidney associated with polycythemia: A case report." in: Oncology letters, Vol. 11, Issue 1, pp. 352-354, (2016) (PubMed).

Zhang, Zhao, Qu, Che, Lin, Jiang, Zhao, Wang: "Expression of S100B during the innate immune of corneal epithelium against fungi invasion." in: International journal of ophthalmology, Vol. 9, Issue 2, pp. 191-7, (2016) (PubMed).

Liu, Zhang, Zhao, Cui, Cao, Guo: "Effects of hypothermia on S100B and glial fibrillary acidic protein in asphyxia rats after cardiopulmonary resuscitation." in: Cell biochemistry and biophysics, Vol. 71, Issue 1, pp. 401-6, (2015) (PubMed).

Sun, Li, Ma, Liu, Xu, Ye, Hou, Wang, Li, Jiang: "The predictive value and potential mechanisms of miRNA-328 and miRNA-378 for brain metastases in operable and advanced non-small-cell lung cancer." in: Japanese journal of clinical oncology, Vol. 45, Issue 5, pp. 464-73, (2015) (PubMed).

Chen, Zhang, Liu, He, Zhou, Shao, Sun, Cao, Gong, Jiang: "A fibrin matrix promotes the differentiation of EMSCs isolated from nasal respiratory mucosa to myelinating phenotypical Schwann-like cells." in: Molecules and cells, Vol. 38, Issue 3, pp. 221-8, (2015) (PubMed).

Tang, Li, Cheng, Huang, Chen: "Effects of diethylstilbestrol on the proliferation and tyrosinase activity of cultured human melanocytes." in: Biomedical reports, Vol. 3, Issue 4, pp. 499-502, (2015) (PubMed).

Wülfing, Günther: "Dendritic cells and macrophages neurally hard-wired in the lymph node." in: Scientific reports, Vol. 5, pp. 16866, (2015) (PubMed).

Jin, Chen, Liu, Liang, Zhang, Zhang: "The expression of endothelial barrier antigen (EBA) and S100B in the rat parietal cortex following brain irradiation." in: Brain research, Vol. 1558, pp. 84-9, (2014) (PubMed).

Tang, Li, Cheng, Jing: "Primary culture of human face skin melanocytes for the study of hyperpigmentation." in: Cytotechnology, Vol. 66, Issue 6, pp. 891-8, (2014) (PubMed).

Lai, Wang, Ling, Wu, Zeng: "Graft of a tissue-engineered neural scaffold serves as a promising strategy to restore myelination after rat spinal cord transection." in: Stem cells and development, Vol. 23, Issue 8, pp. 910-21, (2014) (PubMed).

Zhao, He, Zhang, Tian, Zhao, Yu, Bai: "Electric stimulation and decimeter wave therapy improve the recovery of injured sciatic nerves." in: Neural regeneration research, Vol. 8, Issue 21, pp. 1974-84, (2014) (PubMed).

Qu, Liang, Gu, Liu: "Quercetin alleviates high glucose-induced Schwann cell damage by autophagy." in: Neural regeneration research, Vol. 9, Issue 12, pp. 1195-203, (2014) (PubMed).

Jiang, Qu, Han, Liu, Zhang: "Establishment of immortalized Schwann cells derived from rat embryo dorsal root ganglia." in: International journal of molecular medicine, Vol. 30, Issue 3, pp. 480-6, (2012) (PubMed).

Details zu S100B

Target: S100B (S100 Calcium Binding Protein B (S100B))

Andere Bezeichnung: S100B

Hintergrund:

Synonyms: Protein S100-B,S-100 protein beta chain,S-100 protein subunit beta,S100 calcium-binding protein B,S100B,

Tissue Specificity: Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues.

Background: S100 calcium binding protein B or S100B is a protein of the S-100 protein family. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21, however, this gene is located at 21q22.3. S100B is a glial-derived protein that is a well-established biomarker for severity of neurological injury and prognosis for recovery. S100 beta is a calcium-binding protein that is expressed at high levels in brain primarily by astrocytes. Addition of the disulfide-bonded dimeric form of S100 beta to primary neuronal and glial cultures and established cell lines induces axonal extension and alterations in astrocyte proliferation and phenotype, but evidence that S100 beta exerts the same effects in vivo has not been presented. Reeves et al. (1994) demonstrated that the same effects of the S100B protein are exerted in vivo. They found that both astrocytosis and neurite proliferation occurred in transgenic mice expressing elevated levels of S100b. They suggested that these transgenic mice represent a useful model for studies of the role of S100B in glial-neuronal interactions in normal development and function of the brain and for analyzing the significance of elevated levels of the protein in Down syndrome and Alzheimer disease.

Sequence Similarities: Belongs to the S-100 family.

Molekulargewicht: 11 kDa

UniProt: P04271

Pathways: Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteine